Grimm H
Institute for Medical Microbiology and Clinical Chemistry (Laboratory Dr Gaertner), Weingarten.
Drugs. 1987;34 Suppl 1:14-9. doi: 10.2165/00003495-198700341-00004.
Regression analyses to determine the correlation of minimal inhibitory concentrations (MICs) and inhibition zones produced by ofloxacin discs were carried out with 300 freshly isolated cultures of infective organisms (20 strains from each of 15 species). After pilot studies, 5 micrograms loaded discs were chosen. Studies were performed simultaneously by using ICS/DIN and Kirby-Bauer/NCCLS methods on Mueller-Hinton agar. It was found that the correlation becomes poorer with increasing disc loads and when the Kirby-Bauer method is used. Based on preliminary MIC breakpoints of greater than or equal to 2 mg/L and greater than or equal to 8 mg/L and by calculations from regression equations, the following zone interpretations using the NCCLS method are recommended: resistant up to 12mm, intermediate 13 to 15mm, susceptible 16mm or more. The values for the DIN method are: resistant up to 13mm, intermediate 14 to 17mm, susceptible 18mm or more. No major errors were found in zone interpretations. Minor errors were observed in 0.7% when the NCCLS method was used, and in 1.3% with the DIN method.
采用300株新鲜分离的感染性生物体培养物（15个菌种，每种20株）进行回归分析，以确定氧氟沙星纸片产生的最低抑菌浓度（MIC）与抑菌圈之间的相关性。经过预试验，选择了含5微克药物的纸片。在穆勒-欣顿琼脂上，同时采用ICS/DIN法和柯氏-鲍氏/NCCLS法进行研究。结果发现，随着纸片含药量增加以及使用柯氏-鲍氏法时，相关性变差。根据初步的MIC折点（大于或等于2毫克/升和大于或等于8毫克/升）并通过回归方程计算，建议使用NCCLS法时采用以下抑菌圈判读标准：抑菌圈直径达12毫米及以下为耐药，13至15毫米为中介，16毫米及以上为敏感。DIN法的判读标准为：抑菌圈直径达13毫米及以下为耐药，14至17毫米为中介，18毫米及以上为敏感。抑菌圈判读中未发现重大误差。使用NCCLS法时，观察到0.7%的轻微误差；使用DIN法时，轻微误差为1.3%。
