Hu Yang, Liu Lei, Shan Li-Peng, Chen Jiong
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, China.
Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Meishan Campus, Ningbo University, Ningbo, China.
J Fish Dis. 2022 Feb;45(2):349-359. doi: 10.1111/jfd.13561. Epub 2021 Nov 23.
White spot syndrome virus (WSSV) is an important pathogen causing high mortality in the shrimp industry in aquaculture, yet there is no treatment available to date. In order to find a treatment against WSSV infection, this study examined the anti-WSSV activity of eight natural compounds using shrimp larvae as a model. Among the eight compounds, paeoniflorin showed the most obvious anti-WSSV effect, with a maximum protection efficiency of WSSV-infected shrimp >60% at 100 μM. Furthermore, pretreatment and post-treatment experiments revealed that paeoniflorin could prevent and treat WSSV infection in shrimp. The antiviral activity of paeoniflorin in aquaculture water decreased rapidly with time, and the results showed that the stable anti-WSSV activity of paeoniflorin could only remain in water for 1 day. Thus, the dosing pattern of continuous medication changes was evaluated. Obviously, in the model of continuous change of paeoniflorin, WSSV copy numbers in the virus-treated shrimp group still progressively increased, while the virus content in WSSV -treated group continued to decrease. Interestingly, paeoniflorin inhibited horizontal transmission of WSSV to a certain extent. Notably, paeoniflorin significantly increased the expression of antimicrobial peptides of shrimp to resist WSSV. In conclusion, paeoniflorin has the potential to protect shrimp against WSSV.
白斑综合征病毒（WSSV）是水产养殖对虾产业中导致高死亡率的一种重要病原体，但迄今为止尚无可用的治疗方法。为了找到针对WSSV感染的治疗方法，本研究以对虾幼体为模型，检测了八种天然化合物的抗WSSV活性。在这八种化合物中，芍药苷显示出最明显的抗WSSV效果，在100μM时，对感染WSSV的对虾的最大保护效率>60%。此外，预处理和后处理实验表明，芍药苷可以预防和治疗对虾的WSSV感染。芍药苷在养殖水中的抗病毒活性随时间迅速下降，结果表明芍药苷稳定的抗WSSV活性在水中仅能维持1天。因此，评估了连续给药变化的给药模式。显然，在芍药苷连续变化的模型中，病毒处理对虾组的WSSV拷贝数仍在逐渐增加，而WSSV处理组的病毒含量持续下降。有趣的是，芍药苷在一定程度上抑制了WSSV的水平传播。值得注意的是，芍药苷显著增加了对虾抗菌肽的表达以抵抗WSSV。总之，芍药苷具有保护对虾免受WSSV感染的潜力。
