State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, China.
Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Meishan Campus, Ningbo University, Ningbo 315832, China.
Int J Mol Sci. 2021 Mar 26;22(7):3450. doi: 10.3390/ijms22073450.
Rapid production of prawn () under artificial pressure can result in a series of obvious challenges and is vulnerable to serious losses related to aquatic environmental issues and the unrestrained outbreak of white spot syndrome (WSS). However, to date, there are no therapeutic strategies to contain the spread of the virus. Here, we synthesized 27 coumarin derivatives and evaluated their anti-white spot syndrome virus (WSSV) activity in larvae. We demonstrated that electron-withdrawing and electron-giving substituent groups play an important role in reducing toxicity and WSSV replication, respectively. Two coumarin (2-amino-5-oxo-4-(p-tolyl)-4H,5H-pyrano[3,2-c]chromene-3-carbonitrile) and (2-amino-4-(4-chlorophenyl)-5-oxo-4H,5H-pyrano[3,2-c]chromene-3-carbonitrile) were regarded as the most promising anti-WSSV compounds with maximum antiviral response <5% and median effective concentration <10 mg/L. The mortality of WSSV-infected larvae decreased by more than 60% after exposure to and . With continuous immersion of and exchange, the mortality further decreased to 40% at 120 h. Additionally, and are the relatively stable in aquacultural water, making these agents suitable for use in inhibiting WSSV horizontal transmission in static aquaculture systems. These results showed the marked advantages of using and in the shrimp industry, and suggest that they hold potential for the treatment and prevention of WSSV infection in shrimp seedling culture.
在人工压力下快速生产虾会导致一系列明显的挑战，并容易受到与水生环境问题和白斑综合征（WSS）不受控制爆发相关的严重损失。然而，迄今为止，还没有治疗策略来控制病毒的传播。在这里，我们合成了 27 种香豆素衍生物，并在 幼虫中评估了它们抗白斑综合征病毒（WSSV）的活性。我们证明，吸电子和给电子取代基在降低毒性和 WSSV 复制方面分别起着重要作用。两种香豆素 （2-氨基-5-氧代-4-（对甲苯基）-4H,5H-吡喃并[3,2-c]色烯-3-甲腈）和 （2-氨基-4-（4-氯苯基）-5-氧代-4H,5H-吡喃并[3,2-c]色烯-3-甲腈）被认为是最有前途的抗 WSSV 化合物，最大抗病毒反应 <5%，半数有效浓度 <10mg/L。暴露于 和 后，感染 WSSV 的幼虫死亡率降低了 60%以上。随着 和 的持续浸泡交换，120 小时后死亡率进一步降低至 40%。此外， 和 在水产养殖水中相对稳定，这使得这些试剂适合用于抑制静态水产养殖系统中的 WSSV 水平传播。这些结果表明，在虾产业中使用 和 具有显著优势，并表明它们在虾苗养殖中治疗和预防 WSSV 感染方面具有潜力。
