Engineering Research Center for Medicine, Ministry of Education, Harbin University of Commerce, Harbin 150076, PR China.
Engineering Research Center for Medicine, Ministry of Education, Harbin University of Commerce, Harbin 150076, PR China.
Int J Biol Macromol. 2022 Jan 1;194:445-451. doi: 10.1016/j.ijbiomac.2021.11.086. Epub 2021 Nov 20.
Tri-(2,3-dibromopropyl) isocyanate (TBC), a new emerged persistent organic pollutant, is widely used in fields of flame retardant, textile, rubber and plastic with strong hepatotoxicity. Purple Sweet Potato Polysaccharide (PSPP) has antioxidant and hepatoprotective effects. This study aims to answer the scientific question whether PSPP has a protective effect on TBC induced liver injury. The effect of PSPP on the apoptosis of HepG2 cells was detected by MTT assay, the morphological changes were observed by morphological observation, and the apoptosis rate was determined by flow cytometry. The apoptotic genes were detected by qPCR assay, the relevant protein express was detected by western blot. The correlation between proteins and genes in the apoptosis pathway of HepG2 cells was calculated. To further reveal the apoptosis mechanism of TBC hepatotoxicity in vivo, 19 target genes and 14 apoptotic related proteins of inhibiting apoptosis via death receptor and mitochondria were discussed, all the above results proved that PSPP had protective effect on liver injury induced by TBC. This study not only provided a scientific basis for clarifying the mechanism of TBC hepatotoxicity and the protective effect of PSPP, but also generated the new point and method in terms of the prevention in advance and early intervention of diseases caused by environmental pollution.
三（2,3-二溴丙基）异氰酸酯（TBC）是一种新兴的持久性有机污染物，广泛应用于阻燃剂、纺织品、橡胶和塑料等领域，具有很强的肝毒性。紫甘薯多糖（PSPP）具有抗氧化和保肝作用。本研究旨在回答 PSPP 是否对 TBC 诱导的肝损伤具有保护作用这一科学问题。通过 MTT 法检测 PSPP 对 HepG2 细胞凋亡的影响，通过形态学观察观察细胞形态变化，通过流式细胞术检测细胞凋亡率。通过 qPCR 法检测凋亡基因，通过 Western blot 法检测相关蛋白表达。计算凋亡途径中 HepG2 细胞蛋白与基因的相关性。为进一步揭示 TBC 肝毒性的体内凋亡机制，探讨了抑制凋亡通过死亡受体和线粒体的 19 个靶基因和 14 个凋亡相关蛋白，所有上述结果均证明 PSPP 对 TBC 诱导的肝损伤具有保护作用。本研究不仅为阐明 TBC 肝毒性和 PSPP 的保护作用机制提供了科学依据，而且为环境污染引起的疾病的预防和早期干预提供了新的观点和方法。
