Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Agro-Microbial Resource and Development (Ministry of Agriculture), Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
J Virol Methods. 2022 Feb;300:114370. doi: 10.1016/j.jviromet.2021.114370. Epub 2021 Nov 20.
Previous research has established that Lentinula edodes mycovirus HKB (LeV-HKB) and L. edodes partitivirus 1(LePV1) are major mycoviruses identified in L.edodes germplasm. In this paper, two different methods for curing these two dsRNA mycoviruses, ribavirin treatment and mycelial fragmentation, were evaluated for the first time. Mycelial fragmentation was found to resulted in LeV-HKB- and LePV1-cured fungal strains, whereas ribavirin treatment could eliminate LeV-HKB only. Although no LePV1-cured strain was obtained via ribavirin treatment by the end of the experiment, the relative LePV1 concentration in the eighth successive subcultures was lower than that of the untreated control. The culture features of several virus-cured strains had faster mycelial growth rate and higher colony density than the infected ones. It was also suggested that LeV-HKB infection may affect the pigmentation in plate- and bag-cultivated mycelia of L. edodes strain L135.
先前的研究已经确定，香菇病毒 HKB（LeV-HKB）和香菇伴生病毒 1（LePV1）是在香菇种质资源中鉴定到的主要真菌病毒。在本文中，首次评估了两种不同的方法来治疗这两种 dsRNA 真菌病毒，即利巴韦林处理和菌丝体断裂。研究发现，菌丝体断裂会导致 LeV-HKB 和 LePV1 被治愈的真菌菌株，而利巴韦林处理只能消除 LeV-HKB。尽管到实验结束时，通过利巴韦林处理没有获得 LePV1 被治愈的菌株，但第八次连续继代培养中的相对 LePV1 浓度低于未处理的对照。几种病毒治愈菌株的培养特性显示出比感染菌株更快的菌丝生长速度和更高的菌落密度。此外，LeV-HKB 感染可能会影响香菇菌株 L135 的平板和袋栽菌丝体的色素沉着。
