增强蓝藻的辅因子再生以用于光驱动手性醇的合成。
Enhancing cofactor regeneration of cyanobacteria for the light-powered synthesis of chiral alcohols.
作者信息
Fan Jianhua, Zhang Yinghui, Wu Ping, Zhang Xiaoyan, Bai Yunpeng
机构信息
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China; Department of Applied Biology, East China University of Science and Technology, Shanghai 200237, PR China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China; Department of Bioengineering, East China University of Science and Technology, Shanghai 200237, PR China.
出版信息
Bioorg Chem. 2022 Jan;118:105477. doi: 10.1016/j.bioorg.2021.105477. Epub 2021 Nov 10.
Cyanobacteria Synechocystis sp. PCC 6803 was exploited as green cell factory for light-powered asymmetric synthesis of aromatic chiral alcohols. The effect of temperature, light, substrate and cell concentration on substrate conversions were investigated. Under the optimal condition, a series of chiral alcohols were synthesized with conversions up to 95% and enantiomer excess (ee) > 99%. We found that the addition of NaSO and Angeli's Salt increased the NADPH content by 20% and 25%, respectively. As a result, the time to reach 95% substrate conversion was shortened by 12 h, which demonstrated that the NADPH regeneration and hence the reaction rates can be regulated in cyanobacteria. This blue-green algae based biocatalysis showed its potential for chiral compounds production in future.
蓝藻聚球藻属6803株被用作绿色细胞工厂，用于光驱动的芳香族手性醇的不对称合成。研究了温度、光照、底物和细胞浓度对底物转化率的影响。在最佳条件下，合成了一系列手性醇，转化率高达95%，对映体过量值（ee）>99%。我们发现，添加NaSO和安吉利盐分别使NADPH含量提高了20%和25%。结果，达到95%底物转化率的时间缩短了12小时，这表明蓝藻中的NADPH再生以及反应速率可以得到调控。这种基于蓝藻的生物催化在未来生产手性化合物方面显示出了潜力。
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