Wang Q W, Huang P, Yue M, Huang E J, Cai Y S, Liang P D, Yao P P, Zhang Y, Tan W L, Yu R B
D1 Department of Epidemiology, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
Jiangsu Province Hospital, Nanjing 210029, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2021 Jul 10;42(7):1266-1273. doi: 10.3760/cma.j.cn112338-20210126-00065.
To explore the genetic characteristics and evolution of hantavirus carried by rodents in port area of Ningde in Fujian province in the summer of 2020. Rodents were captured in the port area of Ningde, the RNA was extracted from rodent lung tissues and detected by using specific kit. The positive samples were used for whole-genome sequencing of the virus. Bioinformatics software was used for the analysis on the similarity and genetic variation of the sequences. A total of 112 rodents were captured, including 5 and 2 , the positive rate of hantavirus was 6.25% (7/112). By virus gene sequencing, two hantavirus complete genome sequences were obtained (named as FJ35 and FJ36, GenBank accession numbers: MW449188-MW449193). The genetic analysis results showed that the hantavirus detected in positive samples were SEOV and shared 99% nucleotide similarity with hantavirus strains LZSF21 and JX20140581 isolated from Shandong province. Phylogenetic analysis using the maximum likelihood method showed that the hantavirus detected in positive samples belonged to S3 subtype, sharing the same subtype with hantavirus strains Z37 from Zhejiang province, LZSF21 from Shandong province, and zy27 and Gongzhuling 415 from northeastern China. Compared with FJ372, the amino acid variation of N259S was observed at sites 251-264 of nucleoprotein, which might be related to antigenicity. Another variation of Q81R was observed in glycoprotein compared with SEOV 80-39 segment of coded amino acid of international reference strain, which might also cause the change in antigenicity. The high positive rate of hantavirus in rodents in the port area of Ningde- would increase the risk of natural human infection and epidemic in local area. The hantavirus positive rodents in this focus might be from an endemic area in Shandong. It is necessary to strengthen the imported rodent control in the port area of Ningde. The virus detected in 2 positive samples belonged to SEOV subtype Ⅲ and shared high homologies of nucleotides and amino acid sequences with the hantavirus strains in surrounding area. However, some slight variations occurred in glycoprotein and nucleoprotein amino acid sequences, which might cause changes in its antigeniity.
为探究2020年夏季福建省宁德港区啮齿动物携带的汉坦病毒的基因特征及进化情况。在宁德港区捕获啮齿动物，从啮齿动物肺组织中提取RNA，并使用特定试剂盒进行检测。将阳性样本用于病毒全基因组测序。利用生物信息学软件对序列的相似性和基因变异进行分析。共捕获112只啮齿动物，其中黄毛鼠5只、褐家鼠2只，汉坦病毒阳性率为6.25%（7/112）。通过病毒基因测序，获得两条汉坦病毒全基因组序列（命名为FJ35和FJ36，GenBank登录号：MW449188 - MW449193）。遗传分析结果显示，阳性样本中检测到的汉坦病毒为汉城病毒（SEOV），与从山东省分离的汉坦病毒株LZSF21和JX20140581核苷酸相似性达99%。采用最大似然法进行系统发育分析表明，阳性样本中检测到的汉坦病毒属于S3亚型，与浙江省的汉坦病毒株Z37、山东省的汉坦病毒株LZSF21以及中国东北地区的zy27和公主岭415属于同一亚型。与FJ372相比，核蛋白251 - 264位氨基酸发生N259S变异，可能与抗原性有关。与国际参考株SEOV 80 - 39片段编码氨基酸的糖蛋白相比，观察到Q81R变异，也可能导致抗原性改变。宁德港区啮齿动物汉坦病毒阳性率较高，会增加当地人群自然感染和流行的风险。该疫源地汉坦病毒阳性啮齿动物可能来自山东省的一个疫区。有必要加强宁德港区入境啮齿动物的管控。2份阳性样本中检测到的病毒属于Ⅲ型汉城病毒亚型，与周边地区汉坦病毒株核苷酸和氨基酸序列同源性较高。但糖蛋白和核蛋白氨基酸序列出现一些轻微变异，可能导致其抗原性改变。
