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系统分析甘蓝型油菜 CCCH 锌指家族表明，BnRR-TZFs 参与了胁迫抗性。

Systematic analysis of CCCH zinc finger family in Brassica napus showed that BnRR-TZFs are involved in stress resistance.

机构信息

College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China.

Key Laboratory of Crop Epigenetic Regulation and Development in Hunan Province, Changsha, 410128, China.

出版信息

BMC Plant Biol. 2021 Nov 23;21(1):555. doi: 10.1186/s12870-021-03340-8.

DOI:10.1186/s12870-021-03340-8
PMID:34814855
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8609832/
Abstract

BACKGROUND

CCCH zinc finger family is one of the largest transcription factor families related to multiple biotic and abiotic stresses. Brassica napus L., an allotetraploid oilseed crop formed by natural hybridization between two diploid progenitors, Brassica rapa and Brassica oleracea. A systematic identification of rapeseed CCCH family genes is missing and their functional characterization is still in infancy.

RESULTS

In this study, 155 CCCH genes, 81 from its parent B. rapa and 74 from B. oleracea, were identified and divided into 15 subfamilies in B. napus. Organization and syntenic analysis explained the distribution and collinearity relationship of CCCH genes, the selection pressure and evolution of duplication gene pairs in B. napus genome. 44 diploid duplication gene pairs and 4 triple duplication gene groups were found in B. napus of CCCH family and the segmental duplication is attributed to most CCCH gene duplication events in B. napus. Nine types of CCCH motifs exist in B. napus CCCH family members, and motif C-X-C-X-C-X-H is the most common and a new conserved CCH motif (C-X-C-X-H) has been identified. In addition, abundant stress-related cis-elements exist in promoters of 27 subfamily IX (RR-TZF) genes and their expression profiles indicated that RR-TZF genes could be involved in responses to hormone and abiotic stress.

CONCLUSIONS

The results provided a foundation to understand the basic characterization and genes evolution of CCCH gene family in B. napus, and provided potential targets for genetic engineering in Brassicaceae crops in pursuit of stress-tolerant traits.

摘要

背景

富含半胱氨酸的 CCCH 锌指蛋白家族是与多种生物和非生物胁迫相关的最大转录因子家族之一。油菜（Brassica napus L.）是一种异源四倍体油料作物，由两个二倍体祖先白菜（Brassica rapa）和甘蓝（Brassica oleracea）自然杂交形成。油菜 CCCH 家族基因的系统鉴定尚未完成，其功能特征仍处于起步阶段。

结果

本研究在油菜中鉴定到 155 个 CCCH 基因，其中 81 个来自白菜，74 个来自甘蓝，并分为 15 个亚家族。组织和共线性分析解释了 CCCH 基因在油菜基因组中的分布和同源关系、基因对的选择压力和进化。在油菜 CCCH 家族中发现了 44 对二倍体重复基因对和 4 个三倍体重复基因群，油菜 CCCH 基因的大部分重复事件归因于片段重复。油菜 CCCH 家族成员存在 9 种 CCCH 基序，其中 C-X-C-X-C-X-H 基序最为常见，并鉴定到一个新的保守 CCH 基序（C-X-C-X-H）。此外，27 个亚家族 IX（RR-TZF）基因启动子中存在丰富的与应激相关的顺式作用元件，它们的表达谱表明 RR-TZF 基因可能参与激素和非生物胁迫的响应。

结论

本研究结果为理解油菜 CCCH 基因家族的基本特征和基因进化提供了基础，为十字花科作物遗传工程追求抗逆性提供了潜在的目标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/18268ffdcb69/12870_2021_3340_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/0bb291a08a7a/12870_2021_3340_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/b1a28bd7e6ca/12870_2021_3340_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/756d831b835d/12870_2021_3340_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/2db665fc4a96/12870_2021_3340_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/524849b02907/12870_2021_3340_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/38932d615dda/12870_2021_3340_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/c39da21e3793/12870_2021_3340_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/18268ffdcb69/12870_2021_3340_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/0bb291a08a7a/12870_2021_3340_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/b1a28bd7e6ca/12870_2021_3340_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/756d831b835d/12870_2021_3340_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/2db665fc4a96/12870_2021_3340_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/524849b02907/12870_2021_3340_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/38932d615dda/12870_2021_3340_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/c39da21e3793/12870_2021_3340_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc75/8609832/18268ffdcb69/12870_2021_3340_Fig8_HTML.jpg

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