He Xiaoli, Zheng Ruiyi, Chen Yan, Tan Chengfang
College of Landscape Architecture and Art Design, Hunan Agricultural University, Changsha 410128, China.
Orient Science & Technology College, Hunan Agricultural University, Changsha 410128, China.
Plants (Basel). 2025 Jul 14;14(14):2167. doi: 10.3390/plants14142167.
genes play crucial roles in cell-fate determination and body plan specification during early embryogenesis. However, the specific gene structure and functional differentiation of in is still unclear. We investigated genes in (), (), and (), which are polyploidy models with genome triplication after - divergence. In total, 15, 14, and 32 genes were identified in , , and , respectively. Phylogenetic analysis classified () into three classes with conserved domain organization. Synteny analysis indicated that family expansion during allopolyploidization was mainly due to whole-gene and segmental duplications. -element, gene structure, and expression pattern analyses showed high conservation within the same group. RNA-seq and qRT-PCR results divided into three classes with distinct expression patterns: Class I exhibited moderate and specific expression in buds and inflorescence tips; Class III showed specific low expression in seeds and stamens; while the second class showed expression in most tissues. Sub-cellular localization results showed that the three candidate genes from the three classes exhibited distinct subcellular localizations, with BnSTM-C and BnKNAT3a-A predominantly in the nucleus and BnKNATM1-A in the cytoplasm indicating different expression patterns. Collectively, these findings provide a foundation for further functional studies of genes in .
基因在早期胚胎发育过程中的细胞命运决定和身体蓝图规范中起着至关重要的作用。然而，[物种名称]中[基因名称]的具体基因结构和功能分化仍不清楚。我们研究了[物种名称1]、[物种名称2]和[物种名称3]中的[基因名称]基因，它们是在[分化事件]后基因组三倍体化的多倍体模型。在[物种名称1]、[物种名称2]和[物种名称3]中分别鉴定出15个、14个和32个[基因名称]基因。系统发育分析将[基因名称]分为具有保守结构域组织的三类。共线性分析表明，异源多倍体化过程中[基因家族名称]的扩展主要是由于全基因和片段重复。[顺式作用元件名称]元件、基因结构和表达模式分析表明同一组内具有高度保守性。RNA测序和qRT-PCR结果将[基因名称]分为具有不同表达模式的三类：I类在芽和花序顶端表现出中等且特异性的表达；III类在种子和雄蕊中表现出特异性低表达；而第二类在大多数组织中都有表达。亚细胞定位结果表明，来自这三类的三个候选基因表现出不同的亚细胞定位，BnSTM-C和BnKNAT3a-A主要位于细胞核中而BnKNATM1-A位于细胞质中，表明不同的表达模式。总的来说，这些发现为进一步研究[物种名称]中[基因名称]基因的功能提供了基础。
