Department of Neurology, Second Affiliated Hospital, Institute for Neurological Research & Key Laboratory of Cerebral Microcirculation, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, 271000, Shandong, China.
Shandong Provincial Hospital Affiliated To Shandong First Medical University& Shandong Academy of Medical Sciences, Jinan, 250021, Shandong, China.
Mikrochim Acta. 2021 Nov 23;188(12):428. doi: 10.1007/s00604-021-05081-9.
A surface-enhanced Raman scattering (SERS)-based immunoassay with gold nanostars (GNSs) is utilized for determination of the subarachnoid hemorrhage (SAH) biomarker glial fibrillary acidic protein (GFAP) at very low concentration levels, which allows for early diagnosis and guides clinical decision-making to treat SAH-induced complications. The Raman reporter 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) modified on GNSs was selected as the SERS tags. The SERS immunoassay was assembled by SERS tag and GFAP probe-immobilized ITO substrate. Therefore, the level of GFAP can be detected by monitoring the characteristic Raman peak intensity of GFAP-conjugated GNSs at 1332 cm with a very low detection limit. Under optimized conditions, the assay can work in the GFAP concentration range from 1 pg⋅mL to 1 μg⋅mL, with a detection limit as low as 0.54 fg⋅mL. The performance of the SERS immunoassay proven by the detection of GFAP is equivalent to that of the conventional enzyme-linked immunosorbent assay (ELISA). Scheme 1. Schematic illustration of GNSs SERS immunoassay for ultrasensitive dynamic change detection of GFAP. (SAH: Subarachnoid hemorrhage, SCF: Cerebrospinal fluid; GNSs: gold nanostars; SERS: surface-enhanced Raman scattering; GFAP: glial fibrillary acidic protein).
基于金纳米星（GNSs）的表面增强拉曼散射（SERS）免疫分析用于检测极低浓度的蛛网膜下腔出血（SAH）生物标志物胶质纤维酸性蛋白（GFAP），这有助于早期诊断和指导临床决策以治疗 SAH 引起的并发症。在 GNSs 上修饰的拉曼报告分子 5,5'-二硫代双-2-硝基苯甲酸（DTNB）被选为 SERS 标签。SERS 免疫分析通过 SERS 标签和固定在 ITO 基底上的 GFAP 探针组装。因此，通过监测与 GFAP 结合的 GNSs 在 1332cm 处的特征拉曼峰强度，可以检测 GFAP 的水平，检测限非常低。在优化条件下，该测定法可在 1pg⋅mL 至 1μg⋅mL 的 GFAP 浓度范围内工作，检测限低至 0.54fg⋅mL。通过对 GFAP 的检测证明了 SERS 免疫分析的性能与传统的酶联免疫吸附测定（ELISA）相当。方案 1. GNSs SERS 免疫分析用于超灵敏检测 GFAP 动态变化的示意图。（SAH：蛛网膜下腔出血，CSF：脑脊液；GNSs：金纳米星；SERS：表面增强拉曼散射；GFAP：胶质纤维酸性蛋白）。
