State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Key Lab of Cerebral Microcirculation at the Universities of Shandong, Life Science Research Centre of Taishan Medical University, Taishan 271016, China.
Talanta. 2019 Jun 1;198:527-533. doi: 10.1016/j.talanta.2019.02.040. Epub 2019 Feb 10.
Assay technologies capable of detecting biomarker concentrations in unprocessed whole blood samples are fundamental for applications in medical diagnostics. SERS nano-tags integrated magnetic-separation biosensor (MSB) was realized for the first time for immunoassay in whole blood. The reliability and sensitivity of this method rely, in a large extent, on the quality and properties of the SERS nano-tags. The constructed silicacoated Ag SERS nano-tags as labels were used in a rapid and specific MSB immune sensor to detect Matrix Metalloproteinases 9 (MMP-9) in unprocessed blood samples. With fast screening ability and outstanding sensitivity, we anticipate that this method would greatly promote practical application of stroke-based early-stage cancer diagnosis.
用于检测未处理全血样本中生物标志物浓度的分析技术是医学诊断应用的基础。首次实现了基于表面增强拉曼散射（SERS）纳米标签的集成磁分离生物传感器（MSB）用于全血中的免疫测定。该方法的可靠性和灵敏度在很大程度上取决于 SERS 纳米标签的质量和特性。构建的硅涂层 Ag SERS 纳米标签作为标记物，用于快速和特异性的 MSB 免疫传感器，以检测未经处理的血液样本中的基质金属蛋白酶 9（MMP-9）。该方法具有快速筛选能力和出色的灵敏度，我们预计它将极大地促进基于中风的早期癌症诊断的实际应用。
