Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, Pathumthani, Thailand.
Methods Mol Biol. 2022;2411:219-240. doi: 10.1007/978-1-0716-1888-2_13.
For the past several decades, aquaculture all around the world have been retarded by various disease outbreaks caused by many pathogens including parasites, bacteria, and viruses. Apart from being harmful to human health, the emerging diseases also dramatically affect the farm animals such as livestock and aquatic animals. To cope with this problem, one of the effective prophylactic measures is the application of vaccine. However, the traditional vaccines still have some limitations and several drawbacks; thus there is a need for the development of novel advanced vaccine such as chimeric multiepitope vaccine. Based on the current understanding of genomics and immunoproteomics together with the present bioinformatics tools, the researchers can identify the potential targeted epitopes being recognizable by the immune cells. Additionally, another critical point that should be considered for designing the chimeric multiepitope vaccine is the exposure of all those epitopes to the host organism. Thus, selecting an appropriate linker and joining each identified epitope in a suitable site can create the ideal protein structure protruding all the selected epitopes on its surface. Herein, our study would provide the fundamental platform to develop the multiepitope B-cell vaccine for the prevention and control of the aquatic animal disease starting with the epitope prediction until in vivo testing the multiepitope vaccine efficacy.
在过去的几十年中，世界各地的水产养殖业因寄生虫、细菌和病毒等多种病原体引起的各种疾病爆发而受到阻碍。除了对人类健康有害之外，新出现的疾病也对牲畜和水生动物等农场动物产生了巨大影响。为了应对这一问题，有效的预防措施之一是应用疫苗。然而，传统疫苗仍然存在一些局限性和缺点；因此，需要开发新型的先进疫苗，如嵌合多表位疫苗。基于目前对基因组学和免疫蛋白质组学的理解以及当前的生物信息学工具，研究人员可以识别出可被免疫细胞识别的潜在靶向表位。此外，在设计嵌合多表位疫苗时，另一个关键要点是使所有这些表位暴露于宿主生物。因此，选择合适的接头并将每个鉴定的表位连接到合适的位点可以创建理想的蛋白质结构，将所有选定的表位突出在其表面。在此，我们的研究将为开发用于预防和控制水生动物疾病的多表位 B 细胞疫苗提供基础平台，从表位预测开始，直到体内测试多表位疫苗的功效。
