Department of Microbiology, Faculty of Life Sciences, Infectious Diseases and Environmental Health Research Group, University of Ilorin, Ilorin, Nigeria.
Department of Biological Sciences, College of Sciences, Afe Babalola University, Ado-Ekiti, Nigeria.
J Med Virol. 2022 Jun;94(6):2548-2557. doi: 10.1002/jmv.27473. Epub 2021 Nov 29.
In children, the respiratory syncytial virus and adenovirus majorly cause acute respiratory infections. The study evaluated the seroprevalence and conducted the molecular characterization of human respiratory syncytial virus (HRSV) and human adenovirus (HAdV) among children. Venous blood, nasopharyngeal, and oropharyngeal swabs were collected from children presenting with acute respiratory infections in a tertiary health facility in Ado-Ekiti, Nigeria. A serological investigation was carried out on the sera samples for the detection of anti-HRSV immunoglobulin M (IgM), anti-HRSV IgG, anti-HAdV IgM, and anti-HAdV IgG using ELISA (enzyme-linked immunosorbent assay) kits. The amplification and sequencing of HRSV and HAdV were carried out using specific primer pairs that targeted the glycoprotein (G) gene of HRSV and the hexon gene of HAdV, respectively. The seroprevalence of HRSV IgG and IgM was 73% and 7.5%, respectively, while the seroprevalence of HAdV IgG and IgM was 98.5% and 8.5%, respectively. The age of enrolled children, presence of fever, and cough were associated (p < 0.05) with the infection. HRSV subtype B (HRSV-B) (13.3%), and species of HAdV (Mastadenovirus B and C) (11.7%) were detected among the studied population. There was no viral coinfection with both HRSV and HAdV. In infancy and early childhood, HRSV-B, HAdV species B and C are common etiologic agents of respiratory infections as reported in this study. Further studies on molecular characterization of respiratory tract viruses including circulating respiratory syncytial virus and adenovirus are hereby advocated.
在儿童中，呼吸道合胞病毒和腺病毒主要引起急性呼吸道感染。本研究评估了血清流行率，并对尼日利亚阿多-埃基蒂一家三级医疗机构中出现急性呼吸道感染的儿童进行了人类呼吸道合胞病毒（HRSV）和人类腺病毒（HAdV）的分子特征分析。采集静脉血、鼻咽和口咽拭子，来自在尼日利亚阿多-埃基蒂的一家三级医疗机构出现急性呼吸道感染的儿童。使用酶联免疫吸附试验（ELISA）试剂盒对血清样本进行血清学调查，以检测抗 HRSV 免疫球蛋白 M（IgM）、抗 HRSV IgG、抗 HAdV IgM 和抗 HAdV IgG。使用针对 HRSV 糖蛋白（G）基因和 HAdV 六邻体基因的特异性引物对分别对 HRSV 和 HAdV 进行扩增和测序。HRSV IgG 和 IgM 的血清流行率分别为 73%和 7.5%，而 HAdV IgG 和 IgM 的血清流行率分别为 98.5%和 8.5%。纳入儿童的年龄、发热和咳嗽与感染相关（p<0.05）。在研究人群中检测到 HRSV 亚型 B（HRSV-B）（13.3%）和腺病毒属 B 和 C 种（11.7%）。未发现 HRSV 和 HAdV 同时感染。在婴儿和幼儿期，HRSV-B、HAdV 属 B 和 C 是本研究报告的呼吸道感染的常见病原体。提倡进一步开展呼吸道病毒（包括循环呼吸道合胞病毒和腺病毒）的分子特征分析研究。
