Virology Department, "Centre Pasteur du Cameroun", P.O.Box 1274, Yaounde, Cameroon.
Département de Biochimie, Université de Yaoundé 1, BP 812, Yaounde, Cameroon.
Virol J. 2018 Oct 3;15(1):153. doi: 10.1186/s12985-018-1064-x.
Human adenoviruses (HAdVs) cause a wide range of diseases worldwide, including respiratory infections. Studies on HAdV molecular epidemiology are limited in Cameroon. The purpose of this study is to document the different types HAdV circulating in Cameroon in children with acute respiratory infections.
Nasopharyngeal swabs were collected from 811 children under 15 years from 2011 to 2014. The HAdV detection was assessed by semi-quantitative generic PCR r-gene®. The HAdV-positive samples were typed by amplification and sequencing of partial hexon gene and a real-time PCR. Demographic data were collected and analyzed. The infection and hospitalization risk factors were assessed thought the Chi-square test.
A total of 137/220 HAdV-positive samples were amplified successfully. Six species of HAdV (Mastadenovirus A to F) were detected with B (108/220) and C (47/220) being the predominant strains. Hospitalization and age were significantly associated to HAdV-B and HAdV-C respectively. Phylogenetic analysis of HAdV-B3 virus (18) and B7 (5) shows a conserved and a significant temporal stability in relation to the reference sequence (99.1 to 100% of similarity).
This study reported HAdV species and types detected in children with acute respiratory infections in Cameroon between September 2011 and July 2014. These results support further evaluation of the spatio-temporal circulation pattern of HAdV species and types in Cameroon.
人类腺病毒（HAdV）在全球范围内引起多种疾病，包括呼吸道感染。喀麦隆对 HAdV 分子流行病学的研究有限。本研究旨在记录 2011 年至 2014 年间喀麦隆儿童急性呼吸道感染中循环的不同类型 HAdV。
收集了 811 名 15 岁以下儿童的鼻咽拭子。通过半定量通用 PCR r-基因®评估 HAdV 的检测情况。通过扩增和测序部分六邻体基因以及实时 PCR 对 HAdV 阳性样本进行分型。收集和分析人口统计学数据。通过卡方检验评估感染和住院的危险因素。
共成功扩增了 137/220 份 HAdV 阳性样本。共检测到六种 HAdV（Mastadenovirus A 至 F），其中 B（108/220）和 C（47/220）为主要株。住院和年龄与 HAdV-B 和 HAdV-C 分别显著相关。HAdV-B3 病毒（18 株）和 B7（5 株）的系统进化分析显示，与参考序列相比具有保守性和显著的时间稳定性（相似度为 99.1%至 100%）。
本研究报告了 2011 年 9 月至 2014 年 7 月喀麦隆儿童急性呼吸道感染中检测到的 HAdV 种和型。这些结果支持进一步评估 HAdV 种和型在喀麦隆的时空循环模式。
