Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA.
Chem Commun (Camb). 2021 Dec 7;57(97):13202-13205. doi: 10.1039/d1cc05579h.
We demonstrate the facile and robust generation of giant peptide vesicles by using an emulsion transfer method. These robust vesicles can sustain chemical and physical stresses. The peptide vesicles can host cell-free expression reactions by encapsulating essential ingredients. We show the incorporation of another cell-free expressed elastin-like polypeptide into the existing membrane of the peptide vesicles.
我们展示了一种通过乳液传递方法制备巨型肽囊泡的简便而稳健的方法。这些坚固的囊泡可以承受化学和物理压力。肽囊泡可以通过封装必需成分来容纳无细胞表达反应。我们展示了另一种无细胞表达的弹性蛋白样多肽掺入到现有肽囊泡膜中的情况。
