University of Veterinary and Animal Sciences, Department of Wildlife and Ecology, Lahore, Pakistan.
University of Veterinary and Animal Sciences, Faculty of Fisheries and Wildlife, Lahore, Pakistan.
Braz J Biol. 2021 Nov 19;84:e254253. doi: 10.1590/1519-6984.254253. eCollection 2021.
During the present study, specimens were collected from selected sites of Cholistan desert and Kalabagh Game Reserve, Punjab province, Pakistan. Each captured specimen was tagged with voucher number and morphometric measurements were taken. The average snout to vent length was 172.559±1.40 mm and average weight was 92.1±1.30 g. The DNA of Uromastyx hardwickii was amplified and sequenced using 16S rRNA primer set. The obtained DNA sequence has shown reliable and clear species identification. After trimming ambiguous bases, the obtained 16S rRNA fragment was 520 bp while 16S rRNA fragments aligned with closely matched sequence from NCBI comprised of 510 bp. Closely matched sequences of genus Uromastyx were retrieved from NCBI in blast searches. Neighbour-joining tree of genus Uromastyx was constructed based on p-distance using MEGA X. The mean intraspecific variation was 0.095±0.01 while intraspecific variation was ranging from 0-1%. Similarly, interspecific variation of Uromastyx hardwikii with Saara asmussi, Uromastyx alfredschmidti, Uromastyx geyri, Uromastyx thomasi, Uromastyx alfredschmidti was 0-12%, 0-19%, 0-19%, 0-20%, 12-19% respectively. The newly produced DNA was submitted to NCBI and accession number was obtained (MW052563.1). Results of current study provided information about the molecular and morphological identification of Genus Uromastyx. In our recommendation, comprehensive molecular based identification of Pakistan's reptiles is required to report any new or subspecies from country.
在本研究中，标本采集自巴基斯坦旁遮普省的奇利斯坦沙漠和卡拉巴赫游戏保护区的选定地点。每个捕获的标本都附有凭证号，并进行了形态测量。平均吻肛长为 172.559±1.40 毫米，平均体重为 92.1±1.30 克。使用 16S rRNA 引物对扩增并测序了 Uromastyx hardwickii 的 DNA。获得的 DNA 序列显示出可靠且明确的物种鉴定。在修剪模糊碱基后，获得的 16S rRNA 片段长 520 个碱基，而与 NCBI 中匹配序列对齐的 16S rRNA 片段由 510 个碱基组成。在 blast 搜索中，从 NCBI 中检索到与 Uromastyx 属密切匹配的序列。使用 MEGA X 基于 p-距离构建了 Uromastyx 属的邻接树。种内变异平均值为 0.095±0.01，种内变异范围为 0-1%。同样，Uromastyx hardwikii 与 Saara asmussi、Uromastyx alfredschmidti、Uromastyx geyri、Uromastyx thomasi、Uromastyx alfredschmidti 的种间变异分别为 0-12%、0-19%、0-19%、0-20%、12-19%。新产生的 DNA 被提交给 NCBI，并获得了登录号（MW052563.1）。本研究的结果提供了有关 Uromastyx 属的分子和形态鉴定的信息。在我们的建议中，需要对巴基斯坦的爬行动物进行全面的基于分子的鉴定，以报告该国的任何新种或亚种。
