Department of Molecular Microbiology and Immunology, Keck School of Medicine at USC, Los Angeles, California, USA.
Department of Pathology, Keck School of Medicine at USC, Los Angeles, California, USA.
mSphere. 2021 Dec 22;6(6):e0092021. doi: 10.1128/msphere.00920-21. Epub 2021 Nov 24.
We recently reported that the antimicrobial activity of rifabutin against Acinetobacter baumannii is best modeled by the use of RPMI for susceptibility testing. Here, we define the effects of medium on the susceptibility and frequency of resistance emergence in a panel of A. baumannii, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa clinical isolates. Only A. baumannii was hypersusceptible to rifabutin and using a Galleria mellonella infection model. , the frequency of resistance emergence was greater when the bacteria were selected on RPMI versus tryptic soy agar (TSA) or Mueller-Hinton II (MHII) agar plates. However, the frequency of resistance emergence was lower than in the RPMI condition. Rifabutin has been recently described as a potential adjunctive therapy for antibiotic-resistant A. baumannii infections due to hypersensitivity in iron-depleted media, which may more closely mimic an environment. Here, we report that this hyperactivity is specific for A. baumannii, rather than being a general effect for other pathogens.
我们最近报道称，利福布汀对鲍曼不动杆菌的抗菌活性最好通过使用 RPMI 进行药敏试验来建模。在这里，我们定义了介质对一组鲍曼不动杆菌、大肠杆菌、金黄色葡萄球菌、肺炎克雷伯菌和铜绿假单胞菌临床分离株的药敏性和耐药性出现频率的影响。只有鲍曼不动杆菌对利福布汀高度敏感，并且使用金龟子幼虫感染模型。与在胰蛋白酶大豆琼脂（TSA）或 Mueller-Hinton II（MHII）琼脂平板上选择相比，当在 RPMI 上选择细菌时，耐药性出现的频率更高。然而，耐药性出现的频率低于 RPMI 条件下。利福布汀最近被描述为一种潜在的辅助治疗抗生素耐药鲍曼不动杆菌感染的方法，因为在缺铁培养基中具有超敏反应，这可能更接近一种环境。在这里，我们报告称，这种过度活跃性是鲍曼不动杆菌特有的，而不是其他病原体的一般效应。
