Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Department of Veterinary Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Cell Mol Biol (Noisy-le-grand). 2021 Jan 31;67(1):153-158. doi: 10.14715/cmb/2021.67.1.23.
The present study was designed to evaluate the antimicrobial activity of E. globulus leaves in broiler chicks. Total (n=255) day-old chicks were segregated into five groups i.e. Pathogenic E. coli, S. pullorum, S. gallinarum and C. perfringens type A and control negative group. Each bacterial challenged (1x 107 CFU) group was divided into control positive, antibiotic, probiotic and E. globulus group. Experimental birds were exposed to E. coli, S. pullorum, S. gallinarum and C. perfringens type A at different ages. At 35th day of experiment the log reduction for each group was determined. The highest log reduction in E. coli and C. perfringens Type A colonies count were found in E. globulus (3.26) (2.33) treated group followed by antibiotic (2.85) (1.59) and probiotic (2.84) (1.50) respectively. The log reduction in S. pullorum colonies count was highest in E. globulus (2.50) followed by probiotic (2.24) and antibiotic (2.16). The S. gallinarum colonies count log reduction was found highest for antibiotic (2.84) followed by probiotic (2.48) and E. globulus group. The results of in-vivo experiment revealed that ethanolic extract of E. globulus has antibacterial activity and it can be used as a replacement to low level of antibiotics added in poultry feed.
本研究旨在评估蓝桉叶在肉鸡中的抗菌活性。将 255 只 1 日龄雏鸡分为 5 组，即致病性大肠杆菌、鸡白痢、鸡伤寒和 A 型产气荚膜梭菌以及对照组。每组细菌攻毒（1x107CFU）后分为对照组、抗生素组、益生菌组和蓝桉组。实验鸡在不同日龄接触大肠杆菌、鸡白痢、鸡伤寒和 A 型产气荚膜梭菌。在实验的第 35 天，确定每组的对数减少量。蓝桉处理组的大肠杆菌和 A 型产气荚膜梭菌菌落计数对数减少量最高（3.26）（2.33），其次是抗生素（2.85）（1.59）和益生菌（2.84）（1.50）。蓝桉处理组的鸡白痢菌落计数对数减少量最高（2.50），其次是益生菌（2.24）和抗生素（2.16）。抗生素组的鸡伤寒菌落计数对数减少量最高（2.84），其次是益生菌（2.48）和蓝桉组。体内实验结果表明，蓝桉的乙醇提取物具有抗菌活性，可替代禽饲料中添加的低水平抗生素。
