Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Department of Pharmacology and toxicology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Cell Mol Biol (Noisy-le-grand). 2021 Jan 31;67(1):159-164. doi: 10.14715/cmb/2021.67.1.24.
Aim of the present study was to determine the In-vitro antibacterial activity of ethanolic extract of E. globulus leaves against common multidrug resistant poultry pathogens. Phytochemical analysis through HPLC revealed that kaempeferol (7.315min) followed by querecetin (6.655min) and myrecetin (3.655min). Percent area of kaempeferol (6826.88%) was highest, followed by myrecetin (5516.22%) and querecetin (163.748%). Phytochemical investigation of ethanolic extract of E. globulus leaves through GCMS showed highest retention time (min) α-pinene (20.43) and α-terpineol (20.15) accompanied by spathulenol (11.97), piperitone (11.04). The ethanolic extracts of E. globulus leaves showed a highest zone of inhibition against S. pullorum SP6; 20.64± 2.08, E. coli SE 12; 19.75± 2.83, C. perfringens type A (CPM38-01); 19.46± 2.02. The highest level of MIC of E. globulus noted were against S. gallinarum S22; 133.37±53.294, S. gallinarum S1; 130.20±45.10, S. gallinarum S4; 129.47±24.182, S. gallinarum S3; 126.83±72.392. In conclusion, the study confirmed that the ethanolic extract of E. globulus is composed of active ingredients having antibacterial activity and can be referred as an alternate to antibiotics.
本研究旨在确定蓝桉叶乙醇提取物对常见多药耐药禽病病原体的体外抗菌活性。通过 HPLC 进行的植物化学分析表明，山奈酚（7.315min）、槲皮素（6.655min）和杨梅素（3.655min）。山奈酚的面积百分比最高（6826.88%），其次是杨梅素（5516.22%）和槲皮素（163.748%）。通过 GCMS 对蓝桉叶乙醇提取物进行植物化学研究表明，α-蒎烯（20.43min）和α-松油醇（20.15min）的保留时间最高，其次是匙叶桉醇（11.97%）、胡椒酮（11.04%）。蓝桉叶乙醇提取物对 S. pullorum SP6 的抑菌圈最大；20.64±2.08，E. coli SE 12；19.75±2.83，C. perfringens 型 A（CPM38-01）；19.46±2.02。蓝桉叶的最高 MIC 值为 S. gallinarum S22；133.37±53.294，S. gallinarum S1；130.20±45.10，S. gallinarum S4；129.47±24.182，S. gallinarum S3；126.83±72.392。综上所述，该研究证实蓝桉叶乙醇提取物含有具有抗菌活性的活性成分，可以作为抗生素的替代品。
