Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan Key Laboratory of Tropical Oil Crops Biology, Wenchang, Hainan, 571339, People's Republic of China.
Plant Cell Rep. 2022 Feb;41(2):377-393. doi: 10.1007/s00299-021-02814-1. Epub 2021 Nov 24.
We found that overexpression of EgMYB111 and EgMYB157 genes positively regulate the abiotic stress tolerance. MYB family genes are well-known regulators in modulating the abiotic stress-responsive mechanisms in plants. However, lesser is known about the functional roles of oil palm MYB genes. Previously, we found that oil palm MYB genes such as EgMYB111 and EgMYB157 were significantly up-regulated under salinity, cold, and drought stress conditions. In this study, we over-expressed EgMYB111 and EgMYB157 genes separately in Arabidopsis plants. The transgenic Arabidopsis plants expressing EgMYB111 have shown improved tolerance to salinity, cold and drought stress conditions, whereas transgenic Arabidopsis plants expressing EgMYB157 dispalyed improved tolerance to cold and drought stress conditions only. Various biochemical analyses also revealed significant improvement of antioxidant enzyme activities, photosynthetic pigments, net photosynthetic rate, stomatal conductance, and intercellular CO2 concentration in transgenic plants compared to wild-type plants under cold, drought, and salinity stress conditions. Significant up-regulation of various known stress marker genes such as RD22, RD29A, RAB18, COR47, ABA1, ABI1, HAB1 was also noticed in EgMYB111 and EgMYB157 expressing transgenic plants compared to wild-type plants under cold, drought, and salinity stress conditions. Taken together, over-expression of EgMYB111 and/or EgMYB157 significantly improve abiotic tolerance in transgenic Arabidopsis plants, indicating that EgMYB111 and EgMYB157 are the potential candidates for developing abiotic stress-tolerant crops in near future.
我们发现，EgMYB111 和 EgMYB157 基因的过表达正向调节非生物胁迫耐受性。MYB 家族基因是调节植物中非生物胁迫反应机制的众所周知的调节剂。然而，关于油棕 MYB 基因的功能作用知之甚少。此前，我们发现油棕 MYB 基因，如 EgMYB111 和 EgMYB157，在盐胁迫、冷胁迫和干旱胁迫条件下显著上调。在这项研究中，我们分别在拟南芥中过表达 EgMYB111 和 EgMYB157 基因。表达 EgMYB111 的转基因拟南芥植物表现出对盐胁迫、冷胁迫和干旱胁迫条件的耐受性提高，而表达 EgMYB157 的转基因拟南芥植物仅表现出对冷胁迫和干旱胁迫条件的耐受性提高。各种生化分析还表明，与野生型植物相比，在冷胁迫、干旱胁迫和盐胁迫条件下，转基因植物的抗氧化酶活性、光合色素、净光合速率、气孔导度和胞间 CO2 浓度显著提高。在冷胁迫、干旱胁迫和盐胁迫条件下，还观察到 EgMYB111 和 EgMYB157 表达的转基因植物中各种已知应激标记基因，如 RD22、RD29A、RAB18、COR47、ABA1、ABI1、HAB1 的显著上调。总之，EgMYB111 和/或 EgMYB157 的过表达显著提高了转基因拟南芥植物的非生物耐受性，表明 EgMYB111 和 EgMYB157 是未来开发非生物胁迫耐受作物的潜在候选基因。
