Aksaray University, Department of Chemistry, Aksaray, Turkey.
Hacettepe University, Advanced Technologies Application and Research Center, Ankara, Turkey.
Food Chem. 2022 Mar 15;372:131254. doi: 10.1016/j.foodchem.2021.131254. Epub 2021 Sep 28.
Molecularly imprinted polymer based nanofilms specific to melamine were synthesized on quartz crystal microbalance (QCM) electrode surface. Contact angle measurements, atomic force microscopy, ellipsometry and scanning electron microscopy were used for characterizations process. Some of the findings of the study are as follows: pH 6.0 was found as optimal working pH for melamine detection. Prepared MIP QCM electrode showed a linearity of 99.53% in the concentration range of 50-1000 ng/mL. Langmuir-Freundlich hybrid model was the best fitted isotherm for whole concentration range. The performance of MIP QCM electrode was also confirmed by determining of melamine in melamine spiked milk samples. As a conclusion, the results figured out that not only QCM nanosensor for specific melamine detection but also polymerization strategy were classified as an intriguing alternative for developing new melamine sensing platforms. Limit of detection (LOD) and limit of quantification (LOQ) were calculated as 2.3 ng/mL and 7.8 ng/mL, respectively.
基于分子印迹聚合物的纳米薄膜特异性地针对三聚氰胺进行了合成，该薄膜合成在石英晶体微天平（QCM）电极表面。接触角测量、原子力显微镜、椭圆偏振术和扫描电子显微镜用于对其进行特征分析。研究的一些发现如下：在检测三聚氰胺时，发现 pH 值 6.0 为最佳工作 pH 值。制备的 MIP QCM 电极在 50-1000ng/mL 的浓度范围内表现出 99.53%的线性。整个浓度范围内，Langmuir-Freundlich 混合模型是最适合的等温线模型。通过测定三聚氰胺掺杂牛奶样品中的三聚氰胺，也证实了 MIP QCM 电极的性能。总之，结果表明，不仅 QCM 纳米传感器可用于特异性检测三聚氰胺，而且聚合策略也被归类为开发新的三聚氰胺传感平台的一种有趣的替代方法。检测限（LOD）和定量限（LOQ）分别计算为 2.3ng/mL 和 7.8ng/mL。
