Department of Ultrasound, Pudong New Area People's Hospital Affiliated to Shanghai University of Medicine and Health Sciences, Shanghai, 201200, China.
Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China.
J Nanobiotechnology. 2021 Nov 24;19(1):386. doi: 10.1186/s12951-021-01121-x.
The ratio of fPSA/tPSA in the "grey zone" of tPSA with the concentration range between 4 ng/ml and 10 ng/ml is significant for diagnosis of prostate cancer, and highly efficiency quantification of the ratio of fPSA/tPSA remain elusive mainly because of their extremely low concentration in patients' peripheral blood with high biosample complexity.
We presented an interdigitated spiral-based MXene-assisted organic electrochemical transistors (isMOECTs) biosensor for highly sensitive determination of fPSA/tPSA. The combination of MXene and the interdigitated multiple spiral architecture synergistically assisted the amplification of amperometric signal of biosensor with dual functionalizations of anti-tPSA and anti-fPSA.
The ultrasensitivity of the biosensor was enhanced by tunable multiple spiral architecture and MXene nanomaterials; and the sensor exhibited improved detection limit of tPSA and fPSA down to 0.01 pg/ml and acceptable performance of selectivity, repeatability and stability. Moreover, the isMOECTs displayed area under the curve (AUC) value of 0.8138, confirming the potential applications of isMOECTs in clinics.
The merits of isMOECTs biosensor demonstrated the reliability of MXene-assisted organic electrochemical transistor biosensor with multiple interdigitated spiral for ultrasensitive quantification of fPSA/tPSA, suggesting potential current and future point-of-care testing applications.
在总前列腺特异性抗原（tPSA）浓度范围为 4ng/ml 至 10ng/ml 的“灰区”中，游离前列腺特异性抗原（fPSA）与总前列腺特异性抗原（tPSA）的比值对于前列腺癌的诊断具有重要意义，但其比值的高效定量仍然难以实现，主要是因为其在患者外周血中的浓度极低，生物样本复杂性高。
我们提出了一种基于叉指螺旋 MXene 辅助有机电化学晶体管（isMOECTs）的生物传感器，用于高度灵敏地测定 fPSA/tPSA。MXene 和叉指多螺旋结构的结合协同增强了生物传感器的安培信号放大，具有抗 tPSA 和抗 fPSA 的双重功能化。
可调的多螺旋结构和 MXene 纳米材料增强了生物传感器的超灵敏性；该传感器表现出更低的 tPSA 和 fPSA 检测限，可达 0.01pg/ml，并且具有良好的选择性、重复性和稳定性。此外，isMOECTs 的曲线下面积（AUC）值为 0.8138，证实了 isMOECTs 在临床中的潜在应用。
isMOECTs 生物传感器的优点证明了基于 MXene 辅助的多叉指螺旋有机电化学晶体管生物传感器在 fPSA/tPSA 超灵敏定量方面的可靠性，为当前和未来的即时检测应用提供了潜力。
