Department of Urology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Mikrochim Acta. 2023 Jul 7;190(8):284. doi: 10.1007/s00604-023-05814-y.
A spiral interdigitated MXene-assisted field effect transistor (SiMFETs) was proposed for determination of IL-6 in patients with kidney transplantation infection. Our SiMFETs demonstrated enhanced IL-6 detection range of 10 fg/mL-100 ng/mL due to the combination of optimized transistor's structure and semiconducting nanocomposites. Specifically, on one hand, MXene-based field effect transistor drastically amplified the amperometric signal for determination of IL-6; on the other hand, the multiple spiral structure of interdigitated drain-source architecture improved the transconductance of FET biosensor. The developed SiMFETs biosensor demonstrated satisfactory stability for 2 months, and favorable reproducibility and selectivity against other biochemical interferences. The SiMFETs biosensor exhibited acceptable correlation coefficient (R=0.955) in quantification of clinical biosamples. The sensor successfully distinguished the infected patients from the health control with enhanced AUC of 0.939 (sensitivity of 91.7%, specificity of 86.7%). Those merits introduced here may pave an alternative strategy for transistor-based biosensor in point-of-care clinic applications.
一种螺旋交错 MXene 辅助场效应晶体管（SiMFETs）被提出用于检测肾移植感染患者的 IL-6。由于优化的晶体管结构和半导体纳米复合材料的结合，我们的 SiMFETs 展示了增强的 IL-6 检测范围为 10 fg/mL-100 ng/mL。具体来说，一方面，基于 MXene 的场效应晶体管极大地放大了用于测定 IL-6 的电流信号；另一方面，交错漏源结构的多螺旋结构提高了 FET 生物传感器的跨导。所开发的 SiMFETs 生物传感器在 2 个月内表现出令人满意的稳定性，并且对其他生化干扰具有良好的重现性和选择性。SiMFETs 生物传感器在临床生物样本的定量分析中表现出可接受的相关系数（R=0.955）。该传感器成功地区分了感染患者和健康对照组，AUC 提高到 0.939（灵敏度为 91.7%，特异性为 86.7%）。这里介绍的这些优点可能为基于晶体管的生物传感器在即时医疗诊所应用中开辟了一种替代策略。
