Department of Pathology, Amsterdam UMC Locatie VUmc, Amsterdam, The Netherlands
Amsterdam Cardiovascular Sciences, Amsterdam UMC Location VUmc, Amsterdam, The Netherlands.
Open Heart. 2021 Nov;8(2). doi: 10.1136/openhrt-2021-001842.
Myocardial infarction (MI) is associated with mental health disorders, in which neuroinflammation and cerebral microvascular dysfunction may play a role. Previously, we have shown that the proinflammatory factors N-(carboxymethyl)lysine (CML) and NADPH oxidase 2 (NOX2) are increased in the human infarcted heart microvasculature. The aim of this study was to analyse the presence of CML and NOX2 in the cerebral microvasculature of patients with MI.
Brain tissue was obtained at autopsy from 24 patients with MI and nine control patients. According to their infarct age, patients with MI were divided into three groups: 3-6 hours old (phase I), 6 hours-5 days old (phase II) and 5-14 days old (phase III). CML and NOX2 in the microvasculature were studied through immunohistochemical analysis.
We observed a 2.5-fold increase in cerebral microvascular CML in patients with phase II and phase III MI (phase II: 21.39±7.91, p=0.004; phase III: 24.21±10.37, p=0.0007) compared with non-MI controls (8.55±2.98). NOX2 was increased in microvessels in patients with phase II MI (p=0.002) and phase III MI (p=0.04) compared with controls. No correlation was found between CML and NOX2 (r=0.58, p=0.13).
MI coincides with an increased presence of CML and NOX2 in the brain microvasculature. These data point to proinflammatory alterations in the brain microvasculature that may underlie MI-associated mental health disorders.
心肌梗死（MI）与心理健康障碍有关，其中神经炎症和脑微血管功能障碍可能起作用。此前，我们已经表明，促炎因子 N-（羧甲基）赖氨酸（CML）和 NADPH 氧化酶 2（NOX2）在人类梗死心脏微血管中增加。本研究的目的是分析 MI 患者脑微血管中 CML 和 NOX2 的存在。
从 24 例 MI 患者和 9 例对照患者的尸检中获得脑组织。根据梗死年龄，MI 患者分为三组：3-6 小时（I 期）、6 小时-5 天（II 期）和 5-14 天（III 期）。通过免疫组织化学分析研究了微血管中的 CML 和 NOX2。
我们观察到 II 期和 III 期 MI 患者（II 期：21.39±7.91，p=0.004；III 期：24.21±10.37，p=0.0007）脑微血管 CML 增加了 2.5 倍与非 MI 对照组（8.55±2.98）相比。与对照组相比，NOX2 在 II 期 MI （p=0.002）和 III 期 MI 患者的微血管中增加（p=0.04）。CML 与 NOX2 之间无相关性（r=0.58，p=0.13）。
MI 与脑微血管中 CML 和 NOX2 的存在增加同时发生。这些数据表明，脑微血管中的促炎改变可能是 MI 相关心理健康障碍的基础。
