Altered Pattern of Macrophage Polarization as a Biomarker for Severity of Childhood Asthma.

PURPOSE

Asthma causes a substantial morbidity and mortality burden in children and the pathogenesis of childhood asthma is not completely understood. Macrophages are heterogeneous with divergent M1/M2 polarization phenotypes in response to various stimulations during the inflammatory process. We aimed to investigate the pattern of macrophage polarization and its association with severity and exacerbation in asthmatic children.

PATIENTS AND METHODS

Normal and asthmatic children aged 4-18 years were enrolled for 12 months. Children with asthma were further subgrouped according to their severity and the requirement for hospitalization during exacerbations. Clinical data were obtained from medical records. Peripheral blood samples were collected to analyze macrophage polarization, including M1, M2, and subsets, by flow cytometry.

RESULTS

Fifty-one asthmatic cases and 27 normal controls were included in this study. The level of PM-2KCD14 but not PM-2KCD14 was decreased in asthmatic children. The levels of M2a (CCR7CXCR1), M2b (CCR7CD86), and M2c (CCR7CCR2) subsets, but not M1 (CCR7CD86), were increased in asthmatic children. The levels of M1 were decreased, but the levels of M2c were increased, in children with moderate asthma compared to those with mild asthma. The levels of PM-2KCD14 cells and M1 subsets were decreased, but the M2c subset cells were increased in asthmatic children requiring hospitalization during exacerbations.

CONCLUSION

Macrophage polarization may be involved in the pathogenesis of childhood asthma and is a potential biomarker of childhood asthma disease severity.