Kuo Chang-Hung, Tsai Mei-Lan, Li Chung-Hsiang, Hsiao Hui-Pin, Chao Mei-Chyn, Lee Ming-Sheng, Lin Yi-Ching, Hung Chih-Hsing
Ta-Kuo Clinic, Kaohsiung, Taiwan.
Department of Pediatrics, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan.
J Inflamm Res. 2021 Nov 18;14:6011-6023. doi: 10.2147/JIR.S319754. eCollection 2021.
Asthma causes a substantial morbidity and mortality burden in children and the pathogenesis of childhood asthma is not completely understood. Macrophages are heterogeneous with divergent M1/M2 polarization phenotypes in response to various stimulations during the inflammatory process. We aimed to investigate the pattern of macrophage polarization and its association with severity and exacerbation in asthmatic children.
Normal and asthmatic children aged 4-18 years were enrolled for 12 months. Children with asthma were further subgrouped according to their severity and the requirement for hospitalization during exacerbations. Clinical data were obtained from medical records. Peripheral blood samples were collected to analyze macrophage polarization, including M1, M2, and subsets, by flow cytometry.
Fifty-one asthmatic cases and 27 normal controls were included in this study. The level of PM-2KCD14 but not PM-2KCD14 was decreased in asthmatic children. The levels of M2a (CCR7CXCR1), M2b (CCR7CD86), and M2c (CCR7CCR2) subsets, but not M1 (CCR7CD86), were increased in asthmatic children. The levels of M1 were decreased, but the levels of M2c were increased, in children with moderate asthma compared to those with mild asthma. The levels of PM-2KCD14 cells and M1 subsets were decreased, but the M2c subset cells were increased in asthmatic children requiring hospitalization during exacerbations.
Macrophage polarization may be involved in the pathogenesis of childhood asthma and is a potential biomarker of childhood asthma disease severity.
哮喘给儿童带来了巨大的发病和死亡负担，儿童哮喘的发病机制尚未完全明确。巨噬细胞具有异质性，在炎症过程中对各种刺激会呈现不同的M1/M2极化表型。我们旨在研究哮喘儿童巨噬细胞极化模式及其与疾病严重程度和病情加重的关系。
纳入4至18岁的正常儿童和哮喘儿童，观察12个月。哮喘儿童根据病情严重程度以及病情加重期间是否需要住院进一步分组。从病历中获取临床数据。采集外周血样本，通过流式细胞术分析巨噬细胞极化情况，包括M1、M2及其亚群。
本研究纳入了51例哮喘患儿和27例正常对照。哮喘儿童中PM - 2KCD14水平降低，但PM - 2KCD14未降低。哮喘儿童中M2a（CCR7CXCR1）、M2b（CCR7CD86）和M2c（CCR7CCR2）亚群水平升高，但M1（CCR7CD86）水平未升高。与轻度哮喘儿童相比，中度哮喘儿童的M1水平降低，但M2c水平升高。病情加重期间需要住院的哮喘儿童中，PM - 2KCD14细胞和M1亚群水平降低，但M2c亚群细胞增加。
巨噬细胞极化可能参与儿童哮喘的发病机制，是儿童哮喘疾病严重程度的潜在生物标志物。
