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参与林地草莓黄酮类生物合成的MBW复合体组分的鉴定

Identification of MBW Complex Components Implicated in the Biosynthesis of Flavonoids in Woodland Strawberry.

作者信息

Xu Pengbo, Wu Liang, Cao Minghao, Ma Chao, Xiao Kun, Li Yanbang, Lian Hongli

机构信息

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, United States.

出版信息

Front Plant Sci. 2021 Nov 8;12:774943. doi: 10.3389/fpls.2021.774943. eCollection 2021.

DOI:10.3389/fpls.2021.774943
PMID:34819941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8606683/
Abstract

Flavonoids belong to the family of polyphenolic secondary metabolites and contribute to fruit quality traits. It has been shown that MBW complexes (MYB-bHLH-WD40) regulate the flavonoids biosynthesis in different plants, but only a limited number of MBW complexes have been identified in strawberry species in general. In this study, we identified 112 R2R3-MYB proteins in woodland strawberry; 12 of them were found to have potential functions in regulating flavonoids biosynthesis by phylogenetic analysis. qRT-PCR assays showed that , , , , , and mostly expressed at green stage of fruit development, aligned with proanthocyanidins accumulation; and showed higher expression levels at turning and ripe stages, aligned with anthocyanins accumulation. These results suggest that different MYBs might be involved in flavonoids biosynthesis at specific stages. Furthermore, FvMYB proteins were demonstrated to interact with FvbHLH proteins and induce expression from the promoters of and genes, which encode key enzymes in flavonoids biosynthesis. The co-expression of FvMYB and FvbHLH proteins in strawberry fruits also promoted the accumulation of proanthocyanidins. These findings confirmed and provided insights into the biofunction of MBW components in the regulation of flavonoid biosynthesis in woodland strawberry.

摘要

类黄酮属于多酚类次生代谢产物家族，对果实品质性状有贡献。研究表明，MBW复合物（MYB-bHLH-WD40）调控不同植物中的类黄酮生物合成，但总体而言，在草莓属植物中仅鉴定出有限数量的MBW复合物。在本研究中，我们在森林草莓中鉴定出112个R2R3-MYB蛋白；通过系统发育分析发现其中12个在调控类黄酮生物合成方面具有潜在功能。qRT-PCR分析表明， 、 、 、 、 和 在果实发育的绿色阶段大多表达，与原花青素积累一致； 和 在转色期和成熟阶段显示出较高的表达水平，与花青素积累一致。这些结果表明，不同的MYB可能在特定阶段参与类黄酮生物合成。此外，FvMYB蛋白被证明与FvbHLH蛋白相互作用，并诱导编码类黄酮生物合成关键酶的 和 基因启动子的表达。FvMYB和FvbHLH蛋白在草莓果实中的共表达也促进了原花青素的积累。这些发现证实了MBW组分在森林草莓类黄酮生物合成调控中的生物功能，并提供了相关见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/e786ae8d337d/fpls-12-774943-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/542f7ab050f9/fpls-12-774943-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/eb1ad7e5415c/fpls-12-774943-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/829a60f994cc/fpls-12-774943-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/2cad90e83437/fpls-12-774943-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/b74c667be3d8/fpls-12-774943-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/23a0d8d79b33/fpls-12-774943-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/37b904af596c/fpls-12-774943-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/49f42e05b5a9/fpls-12-774943-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/e786ae8d337d/fpls-12-774943-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/542f7ab050f9/fpls-12-774943-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/eb1ad7e5415c/fpls-12-774943-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/829a60f994cc/fpls-12-774943-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/2cad90e83437/fpls-12-774943-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/b74c667be3d8/fpls-12-774943-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/23a0d8d79b33/fpls-12-774943-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/37b904af596c/fpls-12-774943-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/49f42e05b5a9/fpls-12-774943-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6c8/8606683/e786ae8d337d/fpls-12-774943-g009.jpg

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