Bayani Masomeh, Hasanjani Roushan Mohammad Reza, Soleimani Amiri Mohammad Jafar, Yahyapour Yousef, Ebrahimpour Soheil, Akbarzadeh Jelodar Somayeh
Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Caspian J Intern Med. 2021 Fall;12(4):533-538. doi: 10.22088/cjim.12.4.533.
Via hemodialysis, viral infections can be transmitted in patients a new definition of this infection with no increase in liver enzymes, negative HCV-PCR in serum and presence of virus in the liver and peripheral blood mononuclear cell (PBMC) called occult hepatitis C virus (HCV) infection (OCI). We decided to examine the prevalence of occult hepatitis C infection on hemodialysis cases.
The current research is a cross-sectional study on patients with end-stage renal disease (ESRD) who were at three hemodialysis centers in Mazandaran province in Iran during 2012-2014. In this study of 356 patients who were undergoing hemodialysis, 54 patients were excluded due to positive HCV Ab, and the remaining 302 patients were enrolled. The test of all serum samples for HCV-RNA detection of plasma and PBMCs was done by real-time polymerase chain reaction (real-time PCR).
There was a significant association between the duration of dialysis with the prevalence of occult HCV infection (P=0.017). Eight (2.65%) patients were positive for HBs Ag and with OCI, but none of them was infected with both hepatitis C and B obviously. Also among the total number of patients, nine patients tested positive for HCV RT-PCR in PBMC in which one of them was positive for serum HCV RNA PCR and was excluded from the study.
The results showed that eight patients had an OCI. There was not any association found between age and sex with OCI, but there was a significant relationship between the duration of dialysis with the prevalence of OCI.
通过血液透析，病毒感染可在患者中传播，这种感染的新定义为肝酶无升高、血清丙型肝炎病毒聚合酶链反应（HCV-PCR）阴性且肝脏和外周血单个核细胞（PBMC）中存在病毒，称为隐匿性丙型肝炎病毒（HCV）感染（OCI）。我们决定研究血液透析病例中隐匿性丙型肝炎感染的患病率。
本研究为横断面研究，研究对象为2012年至2014年期间在伊朗马赞德兰省三个血液透析中心接受治疗的终末期肾病（ESRD）患者。在这项对356例接受血液透析的患者的研究中，54例因HCV抗体阳性被排除，其余302例患者被纳入研究。通过实时聚合酶链反应（实时PCR）对所有血清样本进行血浆和PBMC的HCV-RNA检测。
透析时间与隐匿性HCV感染患病率之间存在显著关联（P=0.017）。8例（2.65%）患者HBs Ag阳性且患有OCI，但均未同时明显感染丙型肝炎和乙型肝炎。在所有患者中，9例患者PBMC的HCV RT-PCR检测呈阳性，其中1例血清HCV RNA PCR检测呈阳性，被排除在研究之外。
结果显示8例患者患有OCI。未发现年龄和性别与OCI之间存在关联，但透析时间与OCI患病率之间存在显著关系。
