大鼠下颌下腺腺泡中肌醇磷酸形成的肾上腺素能调节。
Adrenergic regulation of formation of inositol phosphates in rat submandibular acini.
作者信息
Doughney C, Dormer R L, McPherson M A
出版信息
Biochem J. 1987 Feb 1;241(3):705-9. doi: 10.1042/bj2410705.
Formation of inositol phosphates in response to adrenergic secretagogues was studied in rat submandibular acini labelled with myo-[2-3H]inositol. Noradrenaline rapidly (within 5 s) increased radioactivity incorporated into inositol 1,4,5-trisphosphate and inositol tetrakisphosphate, with less rapid (within 1 min) increases in inositol 1,3,4-trisphosphate being observed. Inositol polyphosphate formation was less sensitive to noradrenaline than was stimulation of mucin secretion and was mediated by stimulation of alpha- but not beta-adrenergic receptors. The beta-adrenergic agonist isoproterenol, which is a potent stimulator of mucin secretion [McPherson & Dormer (1984) Biochem. J. 224, 473-481] did not increase formation of inositol mono-, bis- or polyphosphates during a 15 min incubation. The results suggest that inositol phosphates do not mediate beta-adrenergic stimulation of mucin secretion in rat submandibular acini. In addition, rat submandibular acinar cells contain a Ca2+ pool which can be mobilized by isoproterenol [McPherson & Dormer (1984) Biochem. J. 224, 473-481], without involvement of inositol polyphosphates as second messengers.
利用标记有肌醇-[2-³H]肌醇的大鼠下颌下腺腺泡研究了对肾上腺素能促分泌剂的反应中肌醇磷酸的形成。去甲肾上腺素迅速（5秒内）增加了掺入肌醇1,4,5-三磷酸和肌醇四磷酸中的放射性，观察到肌醇1,3,4-三磷酸的增加则较缓慢（1分钟内）。肌醇多磷酸的形成对去甲肾上腺素的敏感性低于粘蛋白分泌的刺激，且由α-肾上腺素能受体而非β-肾上腺素能受体的刺激介导。β-肾上腺素能激动剂异丙肾上腺素是粘蛋白分泌的有效刺激剂[麦克弗森和多尔默（1984年）《生物化学杂志》224卷，473 - 481页]，在15分钟孵育期间并未增加肌醇单磷酸、双磷酸或多磷酸的形成。结果表明，肌醇磷酸并不介导大鼠下颌下腺腺泡中β-肾上腺素能对粘蛋白分泌的刺激。此外，大鼠下颌下腺腺泡细胞含有一个可被异丙肾上腺素动员的Ca²⁺池[麦克弗森和多尔默（1984年）《生物化学杂志》224卷，473 - 481页]，而无需肌醇多磷酸作为第二信使参与。
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