Gichuki Duncan Kiragu, Li Qingyun, Hou Yujun, Liu Yuanshuang, Ma Mengxue, Zhou Huimin, Xu Chen, Zhu Zhenfei, Wang Lina, Musila Fredrick Mutie, Wang Qingfeng, Xin Haiping
Core Botanical Gardens/Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
Metabolites. 2021 Oct 28;11(11):741. doi: 10.3390/metabo11110741.
Lam. is used as a medicinal herb and vegetable. Flavonoids are the major components for the therapeutic effects. However, flavonoids constituents and expression profiles of related genes in organs are unknown. Colorimetric assay showed the highest flavonoid concentration in roots compared to the stem and leaf. Widely target-based metabolome analysis allowed tentative identification of 199 compounds in three organs. Flavonols and flavones were the dominant flavonoids subclasses. Among the metabolites, 171 were common in the three organs. Unique accumulation profile was observed in the root while the stem and leaf exhibited relatively similar patterns. In the root, six unique compounds (jaceosidin, licoagrochalcone D, 8-prenylkaempferol, hesperetin 7-O-(6″malonyl) glucoside, aureusidin, apigenin-4'-O-rhamnoside) that are used for medicinal purposes were detected. In total, 18,427 expressed genes were identified from transcriptome of the three organs covering about 60% of annotated genes in genome. Fourteen gene families, including 52 members involved in the main pathway of flavonoids biosynthesis, were identified. Their expression could be found in at least one organ. Most of the genes were highly expressed in roots compared to other organs, coinciding with the metabolites profile. The findings provide fundamental data for exploration of metabolites biosynthesis in and diversification of parts used for medicinal purposes.
Lam.被用作药草和蔬菜。黄酮类化合物是其治疗作用的主要成分。然而，各器官中黄酮类化合物的成分及相关基因的表达谱尚不清楚。比色法显示，与茎和叶相比，根中黄酮类化合物浓度最高。基于广泛靶向的代谢组分析初步鉴定出三个器官中的199种化合物。黄酮醇和黄酮是主要的黄酮类亚类。在这些代谢物中，171种在三个器官中都有。根中观察到独特的积累模式，而茎和叶表现出相对相似的模式。在根中，检测到六种用于药用的独特化合物（雅可西定、甘草查耳酮D、8-异戊烯基山奈酚、橙皮素7-O-(6″-丙二酰)葡萄糖苷、金黄紫堇灵、芹菜素-4'-O-鼠李糖苷）。从三个器官的转录组中总共鉴定出18427个表达基因，覆盖了基因组中约60%的注释基因。鉴定出14个基因家族，包括52个参与黄酮类生物合成主要途径的成员。它们的表达至少在一个器官中可以检测到。与其他器官相比，大多数基因在根中高表达，这与代谢物谱一致。这些发现为探索Lam.中代谢物的生物合成以及药用部位的多样化提供了基础数据。
