School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China; Food Refrigeration and Computerized Food Technology (FRCFT), Agriculture and Food Science Centre, University College Dublin, National University of Ireland, Belfield, Dublin 4, Ireland; ITMO University, Lomonosova Street 9, Saint-Petersburg 191002, Russia.
Food Chem. 2022 Mar 30;373(Pt B):131591. doi: 10.1016/j.foodchem.2021.131591. Epub 2021 Nov 11.
Trace amounts of 17β-estradiol (E2) in food and the environment poses a threat to human health, which has created the demand for sensitive analytical methods to detect E2. In this study, a novel fluorescent aptasensor was developed for sensitive detection of E2 based on double-chain hybridization between carbon quantum dots-labelled with E2 aptamer (CQDs-aptamer) and FeO nanoparticles modified by complementary DNA (FeO-cDNA). Under the optimal conditions, the aptasensor displayed a good linear range of 10-10 M for E2 with the coefficient of determination (R) of 0.996, and a low detection limit of 3.48 × 10 M was obtained. Besides, the aptasensor showed high selectivity and good reproducibility for E2 detection, which was successfully applied to the sensitive detection of E2 in milk as compared with tap water and lake water with satisfactory recoveries from 85.21% to 114.80%, suggesting the great significance of this aptasensor for detecting food contaminants in the food industry.
痕量的 17β-雌二醇（E2）存在于食物和环境中，对人类健康构成了威胁，这就需要开发出灵敏的分析方法来检测 E2。在本研究中，基于双链杂交，开发了一种新型荧光适体传感器，用于灵敏检测 E2。双链杂交由与 E2 适体（CQDs-aptamer）标记的碳量子点和 FeO 纳米粒子修饰的互补 DNA（FeO-cDNA）之间发生。在最佳条件下，该适体传感器对 E2 的线性范围为 10-10 M，相关系数（R）为 0.996，检测限低至 3.48×10 M。此外，该适体传感器对 E2 检测具有高选择性和良好的重现性，与自来水和湖水相比，成功应用于牛奶中 E2 的灵敏检测，回收率从 85.21%到 114.80%，这表明该适体传感器在食品工业检测食品污染物方面具有重要意义。
