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基于自修复和抗冻离子导电水凝胶的人工生物电子舌传感用于感知涩味和苦味。

Self-healable and anti-freezing ion conducting hydrogel-based artificial bioelectronic tongue sensing toward astringent and bitter tastes.

机构信息

Department of Materials Science and Engineering, National Chiao Tung University, Hsinchu, 300, Taiwan; Department of Materials Science and Engineering, National Yang Ming Chiao Tung University, Hsinchu, 300, Taiwan.

Department of Applied Chemistry, National Yang Ming Chiao Tung University, Hsinchu, 300, Taiwan.

出版信息

Biosens Bioelectron. 2022 Feb 15;198:113811. doi: 10.1016/j.bios.2021.113811. Epub 2021 Nov 17.

Abstract

Numerous efforts have been attempted to mimic human tongue since years. However, they still have limitations because of damages, temperature effects, detection ranges etc. Herein, a self-healable hydrogel-based artificial bioelectronic tongue (E-tongue) containing mucin as a secreted protein, sodium chloride as an ion transporting electrolyte, and chitosan/poly(acrylamide-co-acrylic acid) as the main 3D structure holding hydrogel network is synthesized. This E-tongue is introduced to mimic astringent and bitter mouth feel based on cyclic voltammetry (CV) measurements subjected to target substances, which permits astringent tannic acid (TA) and bitter quinine sulfate (QS) to be detected over wide corresponding ranges of 29.3 mM-0.59 μM and 63.8 mM-6.38 μM with remarkable respective sensitivities of 0.2 and 0.12 wt%. Besides, the taste selectivity of this E-tongue is performed in the presence of various mixed-taste chemicals to show its high selective behavior toward bitter and astringent chemicals. The electrical self-healability is shown via CV responses to illustrate electrical recovery within a short time span. In addition, cytotoxicity tests using HeLa cells are performed, where a clear viability of ≥95% verified its biocompatibility. The anti-freezing sensing of E-tongue tastes at -5 °C also makes this work to be useful at sub-zero environments. Real time degrees of tastes are detected using beverages and fruits to confirm future potential applications in food taste detections and humanoid robots.

摘要

多年来,人们一直试图模仿人类的舌头。然而,由于损伤、温度影响、检测范围等因素,它们仍然存在局限性。在此,我们合成了一种基于自愈水凝胶的人工生物电子舌(E-tongue),其中包含黏蛋白作为分泌蛋白、氯化钠作为离子传输电解质以及壳聚糖/聚丙烯酰胺-共-丙烯酸作为主要的三维结构保持水凝胶网络。该 E-tongue 基于循环伏安法(CV)测量引入了对涩味和苦味的模拟,可检测到广泛的对应范围为 29.3 mM-0.59 μM 和 63.8 mM-6.38 μM 的涩味单宁酸(TA)和苦味硫酸奎宁(QS),其相应的灵敏度分别为 0.2 和 0.12 wt%。此外,该 E-tongue 的味觉选择性在各种混合味觉化学物质存在下进行,以显示其对苦味和涩味化学物质的高选择性行为。通过 CV 响应显示的电自修复能力表明,在短时间内即可实现电恢复。此外,还使用 HeLa 细胞进行了细胞毒性测试,细胞活力≥95%,证明了其生物相容性。E-tongue 味觉在-5°C 的抗冻感也使其在零下环境中有用。使用饮料和水果检测实时味觉,以确认其在食品味觉检测和仿人机器人方面的未来潜在应用。

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