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使用扫描电子显微镜(SEM)、X射线衍射仪(XRD)和傅里叶变换红外光谱仪(FTIR)对SDF、APF、NaF作用于脱矿人乳牙釉质进行定量分析及效果研究

Quantitative Analysis and Effect of SDF, APF, NaF on Demineralized Human Primary Enamel Using SEM, XRD, and FTIR.

作者信息

Jabin Zohra, Nasim Iffat, Vishnu Priya V, Agarwal Nidhi

机构信息

Department of Pedodontics and Preventive Dentistry, Institute of Dental Studies and Technologies, Ghaziabad, Uttar Pradesh, India.

Department of Conservative Dentistry and Endodontics, Saveetha Dental College, Saveetha University, Chennai, Tamil Nadu, India.

出版信息

Int J Clin Pediatr Dent. 2021 Jul-Aug;14(4):537-541. doi: 10.5005/jp-journals-10005-1988.

Abstract

INTRODUCTION

Topical fluoride therapy has proven benefits in the prevention of demineralization. Tooth enamel has shown a great potential for remineralization with an application of topical fluorides if administered at an appropriate time. In an effort to find an effective remineralizing agent, a novel fluoride agent silver diamine fluoride (SDF) has emerged as a potent caries arresting as well as caries preventing agent.

AIM AND OBJECTIVE

The present study was aimed at determining the primary tooth enamel resistance to demineralization after topical application of three fluoride agents SDF, APF, and NaF.

MATERIALS AND METHODS

Enamel specimens were prepared from 40 caries-free primary molars. These specimens were randomly allocated into three groups of 10 specimens each and they were treated by different topical fluorides namely: Group I-SDF, group II-Acidulated phosphate fluoride (APF), group III-Sodium fluoride. Three enamel specimens from each group were placed on custom-made acrylic blocks with 5 × 5 mm of an exposed window for scanning electron microscope (SEM) evaluation and rest of the specimens were ground into a fine powder for X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analysis. The tooth blocks and treated samples were subjected to the demineralization process for 168 hours. They were then qualitatively assessed to evaluate their resistance to demineralization using SEM, XRD, and FTIR.

RESULTS AND CONCLUSION

Morphologically, the enamel of all groups specimens was mostly smooth with some groves and microporosities. Chemically, the Ca/P molar ratios of all groups were similar with slight variations. Structurally, the crystalline phases found in enamel by powder XRD were hydroxyapatite and carbonate apatite; and there was a higher amount of incorporated type B carbonate than type A carbonate as evidenced by FTIR. The study concludes that topical application of a 38% SDF solution can inhibit demineralization of enamel.

HOW TO CITE THIS ARTICLE

Jabin Z, Nasim I, Vishnu Priya V, Quantitative Analysis and Effect of SDF, APF, NaF on Demineralized Human Primary Enamel Using SEM, XRD, and FTIR. Int J Clin Pediatr Dent 2021;14(4):537-541.

摘要

引言

局部用氟疗法已被证实对预防牙釉质脱矿有益。如果在适当的时候使用局部氟化物,牙釉质在再矿化方面具有很大潜力。为了找到一种有效的再矿化剂,一种新型氟化物——氟化银氨(SDF)已成为一种有效的防龋和预防龋齿的药物。

目的

本研究旨在确定局部应用三种氟化物——SDF、APF和NaF后乳恒牙釉质对脱矿的抵抗力。

材料与方法

从40颗无龋乳磨牙制备釉质标本。这些标本被随机分为三组,每组10个标本,并分别用不同的局部氟化物进行处理,即:第一组——SDF,第二组——酸蚀磷酸盐氟化物(APF),第三组——氟化钠。每组的三个釉质标本被放置在定制的丙烯酸块上,有一个5×5毫米的暴露窗口用于扫描电子显微镜(SEM)评估,其余标本被磨成细粉用于X射线衍射(XRD)和傅里叶变换红外光谱(FTIR)分析。牙块和处理后的样本进行168小时的脱矿过程。然后使用SEM、XRD和FTIR对它们进行定性评估,以评估其抗脱矿能力。

结果与结论

形态学上,所有组标本的釉质大多光滑,有一些凹槽和微孔。化学上,所有组的钙/磷摩尔比相似,略有变化。结构上,粉末XRD在釉质中发现的晶相为羟基磷灰石和碳酸磷灰石;FTIR表明,掺入的B型碳酸盐比A型碳酸盐含量更高。该研究得出结论,局部应用38%的SDF溶液可抑制釉质脱矿。

如何引用本文

贾宾·Z、纳西姆·I、毗湿奴·普里娅·V,使用SEM、XRD和FTIR对SDF、APF、NaF对脱矿人乳牙釉质的定量分析及影响。《国际临床儿科牙科杂志》2021年;14(4):537 - 541。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b0b/8585897/fd76cd9d110e/ijcpd-14-537-g001.jpg

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