Kale Yogesh J, Misal Sagar, Dadpe Mahesh V, Dahake Prasanna T, Kendre Shrikant B, Jain Mukul
Department of Pediatric and Preventive Dentistry, Maharashtra Institute of Dental Sciences & Research (Dental College) (MIDSR), Latur, Maharashtra, India.
Private Practitioner, Mumbai, Maharashtra, India.
Int J Clin Pediatr Dent. 2022 Nov-Dec;15(6):643-651. doi: 10.5005/jp-journals-10005-2454.
To investigate the cariostatic and remineralizing effect of two commercial silver diamine fluoride (SDF) preparations on enamel and dentinal caries using a bacterial plaque model.
A total of 32 extracted primary molars were divided into two groups ( = 16), group I (FAgamin), and group II (SDF). Plaque bacterial model was used to induce caries on enamel and dentin. Preoperative evaluation of samples was done using confocal laser microscopy (CLSM) and energy-dispersive X-ray spectroscopy-scanning electron microscope (EDX-SEM). All samples were treated with test materials and evaluated for postoperative remineralization quantification.
Energy-dispersive X-ray spectroscopy (EDX) revealed that mean preoperative levels (in weight %) of silver (Ag) and fluoride (F) in carious enamel lesions were 0.0 and 0.0, which increased postoperatively to 11.40 and 31.05 for FAgamin and 13.61 and 31.87 for SDF, respectively. For dentinal caries, EDX revealed mean preoperative levels (in weight %) of Ag and F were 0.0 and 0.0, which increased to 11.47 and 48.71 for FAgamin and 10.16 and 47.82 for SDF, respectively postoperatively. Both the groups showed evident demineralization with exposed collagen under SEM. The mean value of enamel lesion depth for the group I and II were 38.64 and 39.30 µm, that reduced to 28.02 and 28.70 µm while for dentinal caries, the mean depth from 38.05 and 38.29 µm that reduced significantly to 28.96 and 30.10 µm, respectively ( < 0.001). Caries depth declined significantly after the application of both FAgamin and SDF ( < 0.001).
FAgamin and SDF show similar cariostatic and remineralization potential for dental caries. The bacterial plaque model used in this study is an efficient method to induce artificial carious lesions in teeth.
A comparative evaluation of these two cariostatic and remineralizing agents will aid in identifying the efficacy of both commercial products in treating initial caries lesions in an effective noninvasive and child-friendly manner.
Kale YJ, Misal S, Dadpe MV, Comparison of Cariostatic and Remineralizing Potential of Two Commercial Silver Diamine Fluoride Preparations using Confocal Laser Microscopy and EDX-SEM Spectroscopy: An study. Int J Clin Pediatr Dent 2022;15(6):643-651.
使用细菌菌斑模型研究两种市售氟化亚锡（SDF）制剂对釉质龋和牙本质龋的防龋及再矿化作用。
总共32颗拔除的乳牙被分为两组（每组n = 16），第一组（FAgamin）和第二组（SDF）。使用菌斑细菌模型在釉质和牙本质上诱导龋齿。术前使用共聚焦激光显微镜（CLSM）和能量色散X射线光谱-扫描电子显微镜（EDX-SEM）对样本进行评估。所有样本均用测试材料处理，并对术后再矿化进行定量评估。
能量色散X射线光谱（EDX）显示，龋损釉质病变中银（Ag）和氟（F）的术前平均水平（重量百分比）分别为0.0和0.0，术后FAgamin组分别增至11.40和31.05，SDF组分别增至13.61和31.87。对于牙本质龋，EDX显示Ag和F的术前平均水平（重量百分比）分别为0.0和0.0，术后FAgamin组分别增至11.47和48.71，SDF组分别增至10.16和47.82。两组在扫描电子显微镜下均显示出明显的脱矿，胶原暴露。第一组和第二组釉质病变深度的平均值分别为38.64和39.30 µm，分别降至28.02和28.70 µm；而对于牙本质龋，平均深度从38.05和38.29 µm分别显著降至28.96和30.10 µm（P < 0.001）。应用FAgamin和SDF后龋损深度均显著下降（P < 0.001）。
FAgamin和SDF对龋齿显示出相似的防龋和再矿化潜力。本研究中使用的细菌菌斑模型是在牙齿中诱导人工龋损的有效方法。
对这两种防龋和再矿化剂进行比较评估，将有助于确定这两种市售产品以有效、无创且对儿童友好的方式治疗早期龋损的疗效。
Kale YJ, Misal S, Dadpe MV, 使用共聚焦激光显微镜和EDX-SEM光谱比较两种市售氟化亚锡制剂的防龋和再矿化潜力：一项体外研究。《国际临床儿科牙科学杂志》2022；15(6):643 - 651。
