Department of Animal Science, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Arch Razi Inst. 2021 Sep 1;76(3):553-559. doi: 10.22092/ari.2020.351865.1541. eCollection 2021 Summer.
The current study evaluated the effects of cryopreservation medium supplementation with folic acid as an antioxidant on post-thawed semen quality in bulk. Semen samples were collected from four proved Iranian Mahabadi bulls and diluted in extender containing 1.5% soybean lecithin. The diluted semen was assigned into six parts and supplemented with different doses of folic acid as follows: FA0 (extender without folic acid), FA0.05, FA0.1, FA0.2, FA0.4, and FA0.8 (extenders containing 0.05, 0.1, 0.2, 0.4, and 0.8 mM folic acid, respectively). Then, the semen samples were cryopreserved in liquid nitrogen. Sperm motility and velocity parameters, membrane integrity, abnormal morphology, viability, and lipid peroxidation were evaluated after thawing. In the results, FA0.05 presented higher (p≤0.05) total motility, progressive motility, membrane integrity, and viability and lower lipid peroxidation compared to other groups. Abnormal morphology was not affected (p>0.05) by treatments. In conclusion, supplementation of cryopreservation medium with 0.05 mM folic acid is a helpful method to conserve the quality of post-thawed semen in bulk.
本研究评估了在 bulk 中冷冻保存液中添加叶酸作为抗氧化剂对解冻后精液质量的影响。从 4 头伊朗 Mahabadi 公牛中采集精液样本，并在含有 1.5%大豆卵磷脂的稀释液中稀释。将稀释后的精液分为六份，并添加不同剂量的叶酸，如下所示：FA0（不含叶酸的稀释液）、FA0.05、FA0.1、FA0.2、FA0.4 和 FA0.8（分别含有 0.05、0.1、0.2、0.4 和 0.8mM 叶酸的稀释液）。然后，将精液样品在液氮中冷冻保存。解冻后评估精子活力和速度参数、膜完整性、异常形态、活力和脂质过氧化。结果表明，与其他组相比，FA0.05 组的总活力、前向运动活力、膜完整性和活力更高（p≤0.05），脂质过氧化水平更低。处理对异常形态没有影响（p>0.05）。总之，在冷冻保存液中添加 0.05mM 叶酸是一种有助于保持 bulk 解冻后精液质量的方法。
