Microbiology Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran.
Molecular Biology Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran.
Arch Razi Inst. 2021 Sep 1;76(3):691-697. doi: 10.22092/ari.2020.343573.1509. eCollection 2021 Summer.
is implicated in the etiology of some diseases including fatal enterotoxaemia. Determining dominant toxin types of this microorganism can be helpful in epidemiologic surveys and the formulation of more proper vaccines. To understand the pathogenicity of this bacterium, it seems necessary to describe the toxin and virulence genes content of strains involved in enterotoxaemia and other associated diseases. The current study aimed to isolate and type the toxins of in sheep with suspected enterotoxaemia in Fars province by culture-PCR and ELISA methods and to compare them to isolates of a healthy group. Samples of intestinal contents were collected from enterotoxaemia cases and a healthy group of sheep. The presence of alpha, beta, and epsilon toxins were evaluated by ELISA method. After culture and isolation of C. perfringens, toxin typing and screening of isolates for the presence of beta-2 and enterotoxin were performed by PCR method. was isolated from 102 of 167 suspected enterotoxaemia cases of sheep and from 22 of 50 healthy sheep. The PCR results showed that type A was the most prevalent toxin type in both groups, but according to ELISA type D was the dominant toxin type in the clinical group. The enterotoxin gene was detected in 10% of all isolates from healthy and suspected group isolates of types A and D. The beta-2 gene was identified in 35% and 63.6% of enterotoxaemia-associated isolates and isolates not associated with disease, respectively. In conclusion, Type D of was the dominant causative organism of fatal enterotoxaemia in sheep in Fars province.
在一些疾病的病因学中涉及，包括致命的肠毒素血症。确定该微生物的优势毒素类型有助于进行流行病学调查和制定更合适的疫苗。为了了解该细菌的致病性，似乎有必要描述与肠毒素血症和其他相关疾病相关的菌株的毒素和毒力基因含量。本研究旨在通过培养-PCR 和 ELISA 方法从法尔斯省疑似肠毒素血症的绵羊中分离和分型，并将其与健康组的分离株进行比较。从肠毒素血症病例和健康绵羊组采集肠内容物样本。通过 ELISA 方法评估 alpha、beta 和 epsilon 毒素的存在。在培养和分离后，通过 PCR 方法对分离株进行毒素分型和 beta-2 和肠毒素的存在进行筛选。从 167 例疑似肠毒素血症绵羊中的 102 例和 50 例健康绵羊中分离出。PCR 结果表明，两组中最常见的毒素类型均为 A 型，但根据 ELISA 类型，临床组的优势毒素类型为 D 型。在健康和疑似组的所有分离株中均检测到肠毒素基因，A 型和 D 型分别为 10%和 10%。beta-2 基因分别在 63.6%和 35%的与肠毒素血症相关的分离株和与疾病无关的分离株中被鉴定。总之，D 型是法尔斯省绵羊致命性肠毒素血症的主要病原体。
