利用CRISPR-Cas原核免疫系统赋予对双生病毒的抗性。
Conferring resistance to geminiviruses with the CRISPR-Cas prokaryotic immune system.
作者信息
Baltes Nicholas J, Hummel Aaron W, Konecna Eva, Cegan Radim, Bruns Aaron N, Bisaro David M, Voytas Daniel F
机构信息
Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA.
出版信息
Nat Plants. 2015 Oct;1(10). doi: 10.1038/nplants.2015.145. Epub 2015 Sep 28.
Abstract
To reduce crop losses due to geminivirus infection, we targeted the bean yellow dwarf virus (BeYDV) genome for destruction with the CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) system. Transient assays using BeYDV-based replicons revealed that CRISPR-Cas reagents introduced mutations within the viral genome and reduced virus copy number. Transgenic plants expressing CRISPR-Cas reagents and challenged with BeYDV had reduced virus load and symptoms, thereby demonstrating a novel strategy for engineering resistance to geminiviruses.
摘要
为减少因双生病毒感染造成的作物损失，我们利用CRISPR-Cas（成簇规律间隔短回文重复序列- CRISPR相关蛋白）系统靶向破坏菜豆黄矮病毒（BeYDV）基因组。使用基于BeYDV的复制子进行的瞬时分析表明，CRISPR-Cas试剂在病毒基因组内引入了突变并降低了病毒拷贝数。表达CRISPR-Cas试剂并受到BeYDV攻击的转基因植物病毒载量和症状减轻，从而证明了一种构建抗双生病毒能力的新策略。
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