Jiang Yonglei, Gong Jiangshiqi, Chen Yi, Hu Binbin, Sun Jianfeng, Zhu Yanmei, Xia Zhenyuan, Zou Congming
Yunnan Academy of Tobacco Agricultural Sciences, Kunming, China 650021.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, PR China.
Iran J Biotechnol. 2021 Jul 1;19(3):e2812. doi: 10.30498/ijb.2021.240460.2812. eCollection 2021 Jul.
Microorganisms play an important role in reducing harmful substances in flue-cured tobacco. Numerous studies have been conducted to degrade nicotine by microorganisms.
The present research deals with the isolation of a potent bacterial strain able to efficiently degrade nicotine and tobacco-specific nitrosamines (TSNAs) in flue-cured tobacco.
Bacterial strain J54, capable of efficiently degrading nicotine and tobacco-specific nitrosamines (TSNAs), was isolated from tobacco leaves and identified. The strain J54 can use nicotine as the sole carbon and nitrogen source and could effectively degrade nicotine while growing in a nicotine isolation medium (NIM) medium.
Compared with the control (CK), the total TSNAs content in the tobacco flue-cured eaves after being sprayed with a solution of the J54 strain was found to decrease by 26.22%. Therein, the degradation rates of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N'-nitrosonornicotine (NNN), N'-nitrosoanatabine (NAT), and N'-nitrosoanabasine (NAB) were 24.01%, 26.27%, 28.6%, and 1.83%, respectively.
Bacterial strain J54, was isolated from tobacco leaves and identified as a bacterium, which is similar to based on its morphological and biochemical characteristics and by phylogenetic analysis based on 16S rRNA gene sequences. To our knowledge, this is the first report of the isolation and characterization of a sp. strain that can efficiently degrade nicotine and TSNAs. The findings pave the way for the application of new biotechnologies for the degradation of nicotine and TSNAs by microorganisms.
微生物在降低烤烟中的有害物质方面发挥着重要作用。已经开展了大量利用微生物降解尼古丁的研究。
本研究旨在分离一种能够有效降解烤烟中尼古丁和烟草特有亚硝胺（TSNAs）的强效菌株。
从烟叶中分离出能够有效降解尼古丁和烟草特有亚硝胺（TSNAs）的J54菌株并进行鉴定。J54菌株能够以尼古丁作为唯一碳源和氮源，并且在尼古丁分离培养基（NIM）中生长时能够有效降解尼古丁。
与对照（CK）相比，喷洒J54菌株溶液后的烤烟烟叶中总TSNAs含量降低了26.22%。其中，4-（N-甲基-N-亚硝基氨基）-1-（3-吡啶基）-1-丁酮（NNK）、N'-亚硝基降烟碱（NNN）、N'-亚硝基新烟草碱（NAT）和N'-亚硝基假木贼碱（NAB）的降解率分别为24.01%、26.27%、28.6%和1.83%。
从烟叶中分离出J54菌株并鉴定为一种细菌，根据其形态和生化特征以及基于16S rRNA基因序列的系统发育分析，该菌株与[具体菌株]相似。据我们所知，这是首次报道分离和鉴定出一种能够有效降解尼古丁和TSNAs的[具体菌株名称]菌株。这些发现为利用微生物降解尼古丁和TSNAs的新生物技术应用铺平了道路。
