用于无菌幼虫的产生的方案，用于无菌动物寄生蠕虫感染研究。
A protocol for generating germ-free larvae for gnotobiotic helminth infection studies.
机构信息
Meakins-Christie Laboratories, Department of Medicine, McGill University Health Centre, Montreal, QC H4A 3J1, Canada.
Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada.
出版信息
STAR Protoc. 2021 Nov 10;2(4):100946. doi: 10.1016/j.xpro.2021.100946. eCollection 2021 Dec 17.
The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free ) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions.
内生于蠕虫物种的微生物是破译宿主-寄生虫相互作用的主要障碍。通过重新利用可逆细菌定植系统，我们生成了无菌（germ-free）幼虫，在感染时保持了无菌小鼠的无菌状态。该方案为受控研究蠕虫-微生物-免疫相互作用提供了有价值的工具。
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