• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于无菌幼虫的产生的方案，用于无菌动物寄生蠕虫感染研究。

A protocol for generating germ-free larvae for gnotobiotic helminth infection studies.

机构信息

Meakins-Christie Laboratories, Department of Medicine, McGill University Health Centre, Montreal, QC H4A 3J1, Canada.

Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada.

出版信息

STAR Protoc. 2021 Nov 10;2(4):100946. doi: 10.1016/j.xpro.2021.100946. eCollection 2021 Dec 17.

DOI:10.1016/j.xpro.2021.100946
PMID:34825215
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8603306/
Abstract

The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free ) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions.

摘要

内生于蠕虫物种的微生物是破译宿主-寄生虫相互作用的主要障碍。通过重新利用可逆细菌定植系统，我们生成了无菌（germ-free）幼虫，在感染时保持了无菌小鼠的无菌状态。该方案为受控研究蠕虫-微生物-免疫相互作用提供了有价值的工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/a8a7917561e9/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/1f07fe9a4dd9/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/630de2160940/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/8550ef81a336/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/5ca7b659209a/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/02341b743aac/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/3fa8e870b210/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/fe48033b20a5/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/a8a7917561e9/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/1f07fe9a4dd9/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/630de2160940/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/8550ef81a336/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/5ca7b659209a/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/02341b743aac/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/3fa8e870b210/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/fe48033b20a5/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d313/8603306/a8a7917561e9/gr7.jpg

相似文献

1
A protocol for generating germ-free larvae for gnotobiotic helminth infection studies.用于无菌幼虫的产生的方案，用于无菌动物寄生蠕虫感染研究。
STAR Protoc. 2021 Nov 10;2(4):100946. doi: 10.1016/j.xpro.2021.100946. eCollection 2021 Dec 17.
2
A simplified protocol for deriving sterile, infectious murine Heligmosomoides polygyrus bakeri larvae.一种简化的方法来获取无菌的、具有感染性的鼠旋毛虫幼虫。
STAR Protoc. 2024 Sep 20;5(3):103144. doi: 10.1016/j.xpro.2024.103144. Epub 2024 Aug 21.
3
Superimposed visceral leishmanial infection aggravates response to Heligmosomoides polygyrus.内脏利什曼病重叠感染加重对旋尾线虫的反应。
Parasit Vectors. 2018 Jul 11;11(1):404. doi: 10.1186/s13071-018-2987-1.
4
Production and analysis of immunomodulatory excretory-secretory products from the mouse gastrointestinal nematode Heligmosomoides polygyrus bakeri.从鼠胃肠道线虫 Heligmosomoides polygyrus bakeri 中生产和分析免疫调节排泄分泌产物。
Nat Protoc. 2014 Dec;9(12):2740-54. doi: 10.1038/nprot.2014.184. Epub 2014 Nov 6.
5
The role of lipid content in the infectivity of third-stage larvae of Heligmosomoides polygyrus.脂质含量在多枝单宫线虫三期幼虫感染性中的作用
J Egypt Soc Parasitol. 1997 Dec;27(3):871-80.
6
Small Intestinal Levels of the Branched Short-Chain Fatty Acid Isovalerate Are Elevated during Infection with Heligmosomoides polygyrus and Can Promote Helminth Fecundity.感染旋毛虫时，小肠中的支链短链脂肪酸异戊酸水平升高，并能促进寄生虫的繁殖。
Infect Immun. 2021 Nov 16;89(12):e0022521. doi: 10.1128/IAI.00225-21. Epub 2021 Aug 30.
7
Host grooming and the transmission strategy of Heligmosomoides polygyrus.宿主梳理行为与多枝旋毛虫的传播策略。
J Parasitol. 1995 Dec;81(6):865-9.
8
Infection with a small intestinal helminth, Heligmosomoides polygyrus bakeri, consistently alters microbial communities throughout the murine small and large intestine.感染小肠寄生虫 Heligmosomoides polygyrus bakeri 会持续改变小鼠小肠和大肠中的微生物群落。
Int J Parasitol. 2020 Jan;50(1):35-46. doi: 10.1016/j.ijpara.2019.09.005. Epub 2019 Nov 22.
9
Cultivation of Heligmosomoides polygyrus: an immunomodulatory nematode parasite and its secreted products.多枝单睾线虫的培养：一种免疫调节性线虫寄生虫及其分泌产物
J Vis Exp. 2015 Apr 6(98):e52412. doi: 10.3791/52412.
10
Helminth species richness in wild wood mice, Apodemus sylvaticus, is enhanced by the presence of the intestinal nematode Heligmosomoides polygyrus.肠道线虫多枝缠绕线虫（Heligmosomoides polygyrus）的存在会增加野生林鼠（Apodemus sylvaticus）体内蠕虫的物种丰富度。
Parasitology. 2009 Jun;136(7):793-804. doi: 10.1017/S0031182009006039.

本文引用的文献

1
Infection with a small intestinal helminth, Heligmosomoides polygyrus bakeri, consistently alters microbial communities throughout the murine small and large intestine.感染小肠寄生虫 Heligmosomoides polygyrus bakeri 会持续改变小鼠小肠和大肠中的微生物群落。
Int J Parasitol. 2020 Jan;50(1):35-46. doi: 10.1016/j.ijpara.2019.09.005. Epub 2019 Nov 22.
2
Parasitic Nematodes Exert Antimicrobial Activity and Benefit From Microbiota-Driven Support for Host Immune Regulation.寄生虫线虫发挥抗菌活性，并受益于微生物群驱动的宿主免疫调节支持。
Front Immunol. 2018 Oct 8;9:2282. doi: 10.3389/fimmu.2018.02282. eCollection 2018.
3
Helminth infection promotes colonization resistance via type 2 immunity.
蠕虫感染通过2型免疫促进定植抗性。
Science. 2016 Apr 29;352(6285):608-12. doi: 10.1126/science.aaf3229. Epub 2016 Apr 14.
4
The Intestinal Microbiota Contributes to the Ability of Helminths to Modulate Allergic Inflammation.肠道微生物群有助于蠕虫调节过敏性炎症的能力。
Immunity. 2015 Nov 17;43(5):998-1010. doi: 10.1016/j.immuni.2015.09.012. Epub 2015 Oct 27.
5
Cultivation of Heligmosomoides polygyrus: an immunomodulatory nematode parasite and its secreted products.多枝单睾线虫的培养：一种免疫调节性线虫寄生虫及其分泌产物
J Vis Exp. 2015 Apr 6(98):e52412. doi: 10.3791/52412.
6
MyD88 signaling inhibits protective immunity to the gastrointestinal helminth parasite Heligmosomoides polygyrus.髓样分化因子88（MyD88）信号传导抑制对胃肠道蠕虫寄生虫多形螺旋线虫的保护性免疫。
J Immunol. 2014 Sep 15;193(6):2984-93. doi: 10.4049/jimmunol.1401056. Epub 2014 Aug 11.
7
Immunity to the model intestinal helminth parasite Heligmosomoides polygyrus.对模式肠道寄生虫旋毛虫的免疫。
Semin Immunopathol. 2012 Nov;34(6):829-46. doi: 10.1007/s00281-012-0347-3. Epub 2012 Oct 11.
8
Alteration of the murine gut microbiota during infection with the parasitic helminth Heligmosomoides polygyrus.感染寄生性蠕虫旋毛虫期间，鼠类肠道微生物组的改变。
Inflamm Bowel Dis. 2010 Nov;16(11):1841-9. doi: 10.1002/ibd.21299.
9
Reversible microbial colonization of germ-free mice reveals the dynamics of IgA immune responses.无菌小鼠可发生可逆转的微生物定殖，揭示 IgA 免疫应答的动态变化。
Science. 2010 Jun 25;328(5986):1705-9. doi: 10.1126/science.1188454.
10
IL-4-producing CD4+ T cells in reactive lymph nodes during helminth infection are T follicular helper cells.蠕虫感染期间反应性淋巴结中产生白细胞介素-4的CD4 + T细胞是滤泡辅助性T细胞。
J Exp Med. 2009 May 11;206(5):1001-7. doi: 10.1084/jem.20090313. Epub 2009 Apr 20.