Department of Urology, Wuxi People's Hospital of Nanjing Medical University, Wuxi, Jiangsu Province, People's Republic of China.
Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, 430060, People's Republic of China.
Int Urol Nephrol. 2022 Jul;54(7):1761-1768. doi: 10.1007/s11255-021-03014-2. Epub 2021 Nov 25.
Studies have demonstrated that allicin may play critical roles in the procession of ischemia-reperfusion(I/R) injury. The purpose of this study was to investigate the protective effects of allicin on renal I/R injury by attenuating oxidative stress and apoptosis.
To establish a model of renal I/R, the right kidney underwent 12 h reperfusion after 45 min ischemia, allicin was administered intraperitoneally at concentrations of 40, 50 or 60 mg/kg. NRK-52E cells were treated with allicin at concentrations of 1, 3 or 5 μM in 24 h hypoxia/ 6 h reoxygenation(H/R) treatments. Indicators of HE, oxidative stress, apoptosis were measured to evaluate the effect of aliicin on renal I/R injury.
Allicin protected renal I/R injury by ameliorating histological injury and decreasing the oxidative stress in renal tissues. Meanwhile, allicin significantly downregulated the expression of Bax and caspase-3, upregulated the expression of Bcl-2 in I/R renal tissues and H/R treated NRK-52E cells.
Allicin may exert anti-apoptotic and antioxidative effects to promote renal function recovery in I/R renal tissues and H/R treated NRK-52E cells. Taken together, allicin may be a potential novel therapy option for future renal injury protection.
研究表明，大蒜素可能在缺血再灌注（I/R）损伤过程中发挥关键作用。本研究旨在通过减轻氧化应激和细胞凋亡来探讨大蒜素对肾 I/R 损伤的保护作用。
建立肾 I/R 模型，右肾缺血 45 分钟后再灌注 12 小时，腹腔内给予大蒜素 40、50 或 60mg/kg。NRK-52E 细胞在 24 小时缺氧/6 小时复氧（H/R）处理中用 1、3 或 5μM 的大蒜素处理。测量 HE、氧化应激、细胞凋亡等指标，以评估大蒜素对肾 I/R 损伤的影响。
大蒜素通过改善组织学损伤和减轻肾组织氧化应激来保护肾 I/R 损伤。同时，大蒜素显著下调 I/R 肾组织和 H/R 处理的 NRK-52E 细胞中 Bax 和 caspase-3 的表达，上调 Bcl-2 的表达。
大蒜素可能通过发挥抗凋亡和抗氧化作用，促进 I/R 肾组织和 H/R 处理的 NRK-52E 细胞中肾功能的恢复。综上所述，大蒜素可能是未来肾损伤保护的一种潜在的新型治疗选择。
