Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, New York, USA.
Immunol Rev. 2022 Mar;306(1):234-243. doi: 10.1111/imr.13039. Epub 2021 Nov 25.
Germinal centers (GCs) are the site of antibody affinity maturation, a fundamental immunological process that increases the potency of antibodies and thereby their ability to protect against infection. GC biology is highly dynamic in both time and space, making it ideally suited for intravital imaging. Using multiphoton laser scanning microscopy (MPLSM), the field has gained insight into the molecular, cellular, and structural changes and movements that coordinate affinity maturation in real time in their native environment. On the other hand, several limitations of MPLSM have had to be overcome to allow full appreciation of GC events taking place across different timescales. Here, we review the technical advances afforded by intravital imaging and their contributions to our understanding of GC biology.
生发中心（GCs）是抗体亲和力成熟的场所，这是一个基本的免疫学过程，可以提高抗体的效力，从而增强其抗感染能力。GC 生物学在时间和空间上都具有高度动态性，非常适合活体成像。使用多光子激光扫描显微镜（MPLSM），该领域深入了解了在其天然环境中实时协调亲和力成熟的分子、细胞和结构变化和运动。另一方面，为了充分了解在不同时间尺度上发生的 GC 事件，必须克服 MPLSM 的几个限制。在这里，我们回顾了活体成像带来的技术进步及其对我们理解 GC 生物学的贡献。
