Soybean Genomics and Improvement Laboratory, USDA Agricultural Research Service, Beltsville, Maryland, USA.
Plant Genetics Research Unit, USDA Agricultural Research Service, University of Missouri, Columbia, Missouri, USA.
Proteomics. 2022 Apr;22(7):e2100143. doi: 10.1002/pmic.202100143. Epub 2022 Jan 17.
Using high throughput tandem mass tag (TMT) based tagging technique, we identified 4172 proteins in three developmental stages: early, mid, and late seed filling. We mapped the identified proteins to metabolic pathways associated with seed filling. The elevated abundance of several kinases was observed from the early to mid-stages of seed filling, indicating that protein phosphorylation was a significant event during this period. The early to late seed filling stages were characterized by an increased abundance of proteins associated with the cell wall, oil, and vacuolar-related processes. Among the seed storage proteins, 7S (β-subunit) and 11S (Gy3, Gy4, Gy5) steadily increased in abundance during early to late stages of seed filling, whereas 2S albumin exhibited a decrease in abundance during the same period. An increased abundance of proteases, senescence-associated proteins, and oil synthesis proteins was observed from the mid to late seed filling stages. The mid to late stages of seed filling was also characterized by a lower abundance of transferases, transporters, Kunitz family trypsin, and protease inhibitors. Two enzymes associated with methionine synthesis exhibited lower abundance from early to late stages. This study unveiled several essential enzymes/proteins related to amino acid and protein synthesis and their accumulation during seed development. All data can be accessed through this link: https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=38784ecbd0854bb3801afc0d89056f84. (Accession MSV000087577).
使用高通量串联质量标签 (TMT) 标记技术，我们在三个发育阶段（早期、中期和晚期种子灌浆）中鉴定了 4172 种蛋白质。我们将鉴定出的蛋白质映射到与种子灌浆相关的代谢途径上。在种子灌浆的早期到中期，观察到几种激酶的丰度升高，表明蛋白质磷酸化是这一时期的一个重要事件。从早期到晚期种子灌浆阶段，与细胞壁、油和液泡相关过程相关的蛋白质丰度增加。在种子贮藏蛋白中，7S（β亚基）和 11S（Gy3、Gy4、Gy5）在早期到晚期灌浆阶段的丰度稳定增加，而 2S 白蛋白在同一时期的丰度下降。从中期到晚期种子灌浆阶段，蛋白酶、衰老相关蛋白和油脂合成蛋白的丰度增加。从中期到晚期种子灌浆阶段，转移酶、转运蛋白、Kunitz 家族胰蛋白酶和蛋白酶抑制剂的丰度也较低。与蛋氨酸合成相关的两种酶从中期到晚期的丰度降低。本研究揭示了几种与氨基酸和蛋白质合成及其在种子发育过程中积累相关的重要酶/蛋白质。所有数据均可通过以下链接访问：https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=38784ecbd0854bb3801afc0d89056f84. (访问号 MSV000087577)。
