Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Food Biotechnology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, Thailand.
Int J Syst Evol Microbiol. 2021 Nov;71(11). doi: 10.1099/ijsem.0.005054.
An aerobic, Gram-stain-positive, endospore-forming, rod-shaped and moderately halophilic strain SKP4-6, was isolated from shrimp paste () collected from Samut Sakhon Province, Thailand. Phylogenetic analysis revealed that strain SKP4-6 belonged to the genus and was most closely related to JCM 11546 (98.6 %), KCTC 3788 (98.6 %) and KCTC 3957 (98.6 %) based on 16S rRNA gene sequence similarity. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain SKP4-6 and its related species were 18.2-19.3 % and 69.84-84.51 %, respectively, which were lower than the threshold recommended for species delineation. The strain grew optimally at 30-40 °C, at pH 7.0 and with 10-15 % (w/v) NaCl. It contained l-Orn-d-Asp in the cell wall peptidoglycan. The DNA G+C content was 44.8 mol%. The major fatty acids were iso-C, anteiso-C and anteiso-C. The predominant isoprenoid quinone was MK-7. Phosphatidylglycerol and diphosphatidylglycerol were present as major polar lipids. Based on this polyphasic approach, digital DNA-DNA relatedness and ANI values, strain SKP4-6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SKP4-6 (=JCM 32624=TISTR 2595).
从泰国萨穆特沙空省采集的虾酱中分离到一株好氧、革兰氏阳性、芽孢形成、杆状和中度嗜盐的菌株 SKP4-6。系统发育分析表明，菌株 SKP4-6 属于 属，与 JCM 11546（98.6%）、KCTC 3788（98.6%）和 KCTC 3957（98.6%）最为密切相关，基于 16S rRNA 基因序列相似性。菌株 SKP4-6 与其相关种之间的数字 DNA-DNA 杂交（dDDH）和平均核苷酸同一性（ANI）值分别为 18.2-19.3%和 69.84-84.51%，均低于种系划分推荐的阈值。该菌株在 30-40°C、pH 7.0 和 10-15%（w/v）NaCl 下生长最佳。细胞壁肽聚糖中含有 l-Orn-d-Asp。DNA G+C 含量为 44.8mol%。主要脂肪酸为 iso-C、anteiso-C 和 anteiso-C。主要的异戊烯醌为 MK-7。磷脂酰甘油和双磷脂酰甘油为主要极性脂。基于多相分析、数字 DNA-DNA 相关值和 ANI 值，菌株 SKP4-6 代表了 属的一个新种，提议命名为 sp. nov.。模式菌株为 SKP4-6（=JCM 32624=TISTR 2595）。
