Wang Kaibiao, Zhang Lei, Yang Yang, Pan Yuanyuan, Meng Lin, Liu Henan, Hong Shan, Huang Haipeng, Jiang Juquan
Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
Int J Syst Evol Microbiol. 2015 Jun;65(Pt 6):1908-1914. doi: 10.1099/ijs.0.000198. Epub 2015 Mar 20.
A Gram-stain-positive, endospore-forming, moderately halophilic bacterial strain, NEAU-ST10-40T, was isolated from a saline and alkaline soil in Anda City, China. It was strictly aerobic, rod-shaped and motile by peritrichous flagella. It formed light yellow colonies and grew at NaCl concentrations of 3-15 % (w/v) (optimum, 8 %, w/v), at pH 7.0-9.0 (optimum, pH 8.0) and at 4-60 °C (optimum, 30 °C). It contained meso-diaminopimelic acid in the cell-wall peptidoglycan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that it belonged to the genus Halobacillus. Levels of 16S rRNA gene sequence similarity between strain NEAU-ST10-40T and the type strains of related species of the genus Halobacillus ranged from 98.8 % (Halobacillus alkaliphilus FP5T) to 97.1 % (Halobacillus kuroshimensis IS-Hb7T). DNA-DNA hybridization relatedness values between strain NEAU-ST10-40T and H. alkaliphilus DSM 18525T, Halobacillus campisalis KCTC 13144T, Halobacillus yeomjeoni DSM 17110T, Halobacillus halophilus DSM 2266T, Halobacillus litoralis DSM 10405T, Halobacillus dabanensis DSM 18199T, Halobacillus salinus DSM 18897T, Halobacillus naozhouensis DSM 21183T, Halobacillus trueperi DSM 10404T and Halobacillus salsuginis DSM 21185T were from 43 ± 1 to 19 ± 1 % (mean ± sd). The DNA G+C content was 39.3 mol%. The major fatty acids (>10 %) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0, the only respiratory quinone detected was MK-7, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and three unknown lipids. On the basis of the data presented, strain NEAU-ST10-40T is considered to represent a novel species, for which the name Halobacillus andaensis sp. nov. is proposed. The type strain is NEAU-ST10-40T ( = CGMCC 1.12153T = DSM 25866T).
从中国安达市盐碱土中分离出一株革兰氏阳性、形成芽孢、中度嗜盐的细菌菌株NEAU-ST10-40T。它严格好氧，杆状，具周生鞭毛，能运动。形成浅黄色菌落，在NaCl浓度为3-15%（w/v）（最适为8%，w/v）、pH 7.0-9.0（最适pH 8.0）和4-60℃（最适30℃）条件下生长。其细胞壁肽聚糖中含有内消旋二氨基庚二酸。基于16S rRNA基因序列的系统发育分析表明，它属于嗜盐芽孢杆菌属。菌株NEAU-ST10-40T与嗜盐芽孢杆菌属相关种模式菌株的16S rRNA基因序列相似性水平在98.8%（嗜碱嗜盐芽孢杆菌FP5T）至97.1%（黑岛嗜盐芽孢杆菌IS-Hb7T）之间。菌株NEAU-ST10-40T与嗜碱嗜盐芽孢杆菌DSM 18525T、盐场嗜盐芽孢杆菌KCTC 13144T、盐沼嗜盐芽孢杆菌DSM 17110T、嗜盐嗜盐芽孢杆菌DSM 2266T、海岸嗜盐芽孢杆菌DSM 10405T、达坂嗜盐芽孢杆菌DSM 181,99T、盐生嗜盐芽孢杆菌DSM 18897T纳潮嗜盐芽孢杆菌DSM 21183T、特鲁珀嗜盐芽孢杆菌DSM 10404T和盐渍嗜盐芽孢杆菌DSM 21185T的DNA-DNA杂交相关值为43±1%至19±1%（平均值±标准差）。DNA G+C含量为39.3 mol%。主要脂肪酸（>10%）为anteiso-C15:0、anteiso-C17:0和iso-C16:0，检测到的唯一呼吸醌为MK-7，极性脂类由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、两种未知磷脂和三种未知脂类组成。根据所提供的数据，菌株NEAU-ST10-40T被认为代表一个新物种，为此提出名为安达嗜盐芽孢杆菌（Halobacillus andaensis sp. nov.）的名称。模式菌株为NEAU-ST10-40T（=CGMCC 1.12153T=DSM 25866T）。
