Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha Nagar, Thandalam, Chennai 602107, India.
Vel Tech High Tech Dr. Rangarajan Dr. Sakunthala Engineering College, Avadi, Chennai 600062, India.
Bioresour Technol. 2022 Feb;346:126405. doi: 10.1016/j.biortech.2021.126405. Epub 2021 Nov 23.
Microbial reduction of black strap molasses (BSM) by Clostridium acetobutylicum MTCC 11,274 was performed for the production of biobutanol. The optimum fermentation conditions were predicted using one factor at a time (OFAT) method. The identification of significant parameters was performed using Plackett-Burman Design (PBD). Furthermore the fermentation conditions were optimized using central composite design (CCD). The kinetics of substrate utilization and product formation were investigated. Initial pH, yeast extract concentration (g/L) and total reducing sugar concentration (g/L) were found as significant parameters affecting butanol production using C. acetobutylicum MTCC11274. The maximum butanol production under optimal condition was 10.27 + 0.82 g/L after 24 h. The waste black strap molasses obtained from sugar industry could be used as promising substrate for the production of next generation biofuel.
利用丙酮丁醇梭菌（Clostridium acetobutylicum MTCC 11,274）对黑糖蜜（BSM）进行微生物还原，以生产生物丁醇。采用单因素法（OFAT）预测最佳发酵条件。利用 Plackett-Burman 设计（PBD）确定显著参数。进一步利用中心复合设计（CCD）对发酵条件进行优化。研究了基质利用和产物形成的动力学。初始 pH 值、酵母提取物浓度（g/L）和总还原糖浓度（g/L）被发现是影响丙酮丁醇梭菌 MTCC11274 生产丁醇的显著参数。在最佳条件下，24 小时后可获得最大丁醇产量为 10.27 ± 0.82 g/L。从制糖工业获得的废黑糖蜜可以作为生产下一代生物燃料的有前途的底物。
