Abou-Zeid A A, Fouad M, Yassein M
Zentralbl Bakteriol Naturwiss. 1978;133(2):125-34. doi: 10.1016/s0323-6056(78)80023-8.
Trials succeeded in raising the efficiencies of the fermentation medium, used in the fermentative production of acetone-butanol by Clostridium acetobutylicum. Egyptian black strap molasses (50.0% sugars) was suitable as carbon source in the fermentation medium, and (NH4)2SO4 was utilized with great success as inorganic nitrogen source. 140.0 g/l black strap molasses (about 7.0% sugars) and 3.0 g/l (NH4)2SO4 were the optimum concentrations for obtaining good yields of acetone and butanol. Molasses and (NH4)2SO4 were preferred because they are cheaper than the other carbon and organic nitrogen sources, used in the fermentative production of acetone-butanol. The percentage increase of the total solvents produced in the fermentation (production medium) was increased by 64.0. The slop (by-product of the acetone-butanol fermentation after distillation) was re-used in the fermentation medium as organic nitrogen source and supported the microorganisms for a good production of acetone and butanol, while when stillage was used in the production medium, the total solvents output was less than that produced in the medium containing slop.
试验成功提高了用于丙酮丁醇梭菌发酵生产丙酮 - 丁醇的发酵培养基的效率。埃及黑糖蜜（含糖量50.0%）适合作为发酵培养基中的碳源，硫酸铵作为无机氮源取得了巨大成功。140.0 g/l黑糖蜜（约含糖7.0%）和3.0 g/l硫酸铵是获得高产丙酮和丁醇的最佳浓度。选择糖蜜和硫酸铵是因为它们比用于丙酮 - 丁醇发酵生产的其他碳源和有机氮源更便宜。发酵（生产培养基）中产生的总溶剂的百分比增加了64.0。酒糟（蒸馏后丙酮 - 丁醇发酵的副产物）作为有机氮源重新用于发酵培养基中，并支持微生物良好地生产丙酮和丁醇，而当在生产培养基中使用釜馏残液时，总溶剂产量低于含有酒糟的培养基中产生的产量。
