Lobov Arseniy A, Babkina Irina Y, Danilov Lavrentii G, Masharskiy Alexey E, Predeus Alexander V, Mikhailova Natalia A, Granovitch Andrei I, Maltseva Arina L
Department of Invertebrate Zoology, St. Petersburg State University, 199034 St. Petersburg, Russia.
Laboratory of Regenerative Biomedicine, Institute of Cytology of the RAS, 194064 St. Petersburg, Russia.
Biology (Basel). 2021 Oct 22;10(11):1087. doi: 10.3390/biology10111087.
Genus subgenus (Mollusca, Caenogastropoda) includes the "obtusata" group of closely related species ( and ). The anatomy of the adult reproductive system (pallial oviduct) is the only reliable feature used for species identification in females of these species. Reproductive system anatomy and reproduction-associated proteins often diverge between sibling species. Despite being of high evolutionary interest, the molecular basis of this divergence remains poorly understood. We performed proteotranscriptomic comparison of oviducts of and by RNA-seq on Illumina HiSeq 2500 and two-dimensional protein electrophoresis (2D DIGE) with MS/MS identification of the species-specific proteins. The interspecies differences in the oviduct were associated with (1) metabolic proteins reflecting overall physiological differences between and , (2) receptor proteins, and (3) transcripts related to transposable elements (TEs). Various receptors identified may recognize a wide variety of ligands from pathogen-associated molecular patterns to specific carbohydrates on the sperm surface. Therefore, these may participate in immune defense as well as in sperm storage and regulation. Species-specificity of multiple TE sequences (coding for reverse transcriptase and ribonuclease H) may indicate the important role of these genomic elements in the species divergence, which has not been reported previously.
属亚属（软体动物门，新腹足目）包括一组关系密切的“钝圆”物种（和）。成年生殖系统（外套膜输卵管）的解剖结构是用于这些物种雌性个体物种鉴定的唯一可靠特征。生殖系统解剖结构和与繁殖相关的蛋白质在亲缘物种之间常常存在差异。尽管具有很高的进化研究价值，但这种差异的分子基础仍知之甚少。我们通过在Illumina HiSeq 2500上进行RNA测序以及二维蛋白质电泳（2D DIGE）并通过MS/MS鉴定物种特异性蛋白质，对和的输卵管进行了蛋白质转录组比较。输卵管中的种间差异与以下方面有关：（1）反映和之间整体生理差异的代谢蛋白，（2）受体蛋白，以及（3）与转座元件（TEs）相关的转录本。鉴定出的各种受体可能识别从病原体相关分子模式到精子表面特定碳水化合物等多种配体。因此，这些受体可能参与免疫防御以及精子储存和调节。多个TE序列（编码逆转录酶和核糖核酸酶H）的物种特异性可能表明这些基因组元件在物种分化中发挥重要作用，而此前尚未有相关报道。
