De-Montijo-Prieto Soumi, Razola-Díaz María Del Carmen, Gómez-Caravaca Ana María, Guerra-Hernandez Eduardo Jesús, Jiménez-Valera María, Garcia-Villanova Belén, Ruiz-Bravo Alfonso, Verardo Vito
Department of Microbiology, Campus of Cartuja, University of Granada, 18071 Granada, Spain.
Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18071 Granada, Spain.
Biology (Basel). 2021 Oct 25;10(11):1091. doi: 10.3390/biology10111091.
In the field of food preservation, encapsulated Essential Oils (EOs) could be the best non-toxic and eco-friendly tool for food preservative applications substituting the chemicals ones that have several disadvantages for the environment and health. Thirteen commercial EOs from plants, fruits, and vegetables were characterized by GC-MS. The antioxidant activity was measured by DPPH and ABTS techniques. Antimicrobial activity was assessed by agar well-diffusion method and the Minimum Inhibitory Concentration (MIC) by agar dilution method against six bacteria, , and . All the EOs tested have demonstrated antioxidant activity in the range of IC 0.01-105.32 mg/mL. Between them, cinnamon EOs were the best, followed by oregano and thyme EOs. Fennel EO showed the lowest radical scavenging. MIC values ranged from 0.14 to 9 mg/mL. , thyme, and oregano EOs were the most effective against the bacterial species tested, and the yeast . On the contrary, citric fruit EOs showed low or no inhibition against most bacterial strains. The percentages of inhibition of mycelia growth of ranged from 3.4 to 98.5%. Thyme, oregano, mint, and fennel EOs showed the highest inhibition.
在食品保鲜领域,包封精油(EOs)可能是食品保鲜应用中最佳的无毒且环保工具,可替代对环境和健康有诸多不利影响的化学保鲜剂。通过气相色谱 - 质谱联用(GC - MS)对来自植物、水果和蔬菜的13种商业精油进行了表征。采用二苯基苦味酰基自由基(DPPH)和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)技术测定抗氧化活性。通过琼脂扩散法评估抗菌活性,并通过琼脂稀释法测定对六种细菌、 和 的最低抑菌浓度(MIC)。所有测试的精油在IC为0.01 - 105.32 mg/mL范围内均表现出抗氧化活性。其中,肉桂精油效果最佳,其次是牛至和百里香精油。茴香精油的自由基清除能力最低。MIC值范围为0.14至9 mg/mL。百里香、牛至和迷迭香精油对测试的细菌种类以及酵母 最为有效。相反,柑橘类水果精油对大多数细菌菌株的抑制作用较低或无抑制作用。 菌丝体生长的抑制率范围为3.4%至98.5%。百里香、牛至、薄荷和茴香精油表现出最高的抑制作用。