Tyumentseva Marina, Mikhaylova Yulia, Prelovskaya Anna, Karbyshev Konstantin, Tyumentsev Aleksandr, Petrova Lyudmila, Mironova Anna, Zamyatin Mikhail, Shelenkov Andrey, Akimkin Vasiliy
Central Research Institute of Epidemiology, Novogireevskaya Str., 3a, 111123 Moscow, Russia.
National Medical and Surgical Center Named after N.I. Pirogov, Nizhnyaya Pervomayskaya Str., 70, 105203 Moscow, Russia.
Antibiotics (Basel). 2021 Oct 26;10(11):1301. doi: 10.3390/antibiotics10111301.
is a member of the ESKAPE opportunistic pathogen group, which includes six species of the most dangerous microbes. This pathogen is characterized by the rapid acquisition of antimicrobial resistance, thus causing major healthcare concerns. This study presents a comprehensive analysis of clinical isolates based on whole-genome sequencing data. The isolate collection studied was characterized by a variety of clonal lineages with a domination of high-risk epidemic clones and different CRISPR/Cas element patterns. This is the first report on the coexistence of two and even three different types of CRISPR/Cas systems simultaneously in Russian clinical strains of . The data include molecular typing and genotypic antibiotic resistance determination, as well as the phylogenetic analysis of the full-length gene and anti-CRISPR genes sequences, predicted prophage sequences, and conducted a detailed CRISPR array analysis. The differences between the isolates carrying different types and quantities of CRISPR/Cas systems were investigated. The pattern of virulence factors in isolates lacking putative CRISPR/Cas systems significantly differed from that of samples with single or multiple putative CRISPR/Cas systems. We found significant correlations between the numbers of prophage sequences, antibiotic resistance genes, and virulence genes in isolates with different patterns of CRISPR/Cas-elements. We believe that the data presented will contribute to further investigations in the field of bacterial pathoadaptability, including antimicrobial resistance and the role of CRISPR/Cas systems in the plasticity of the genome.
是ESKAPE机会致病菌组的成员，该组包括六种最危险的微生物。这种病原体的特点是能迅速获得抗菌抗性，从而引起了医疗保健领域的重大关注。本研究基于全基因组测序数据对临床分离株进行了全面分析。所研究的分离株集合具有多种克隆谱系，其中高风险流行克隆占主导地位，且具有不同的CRISPR/Cas元件模式。这是关于在俄罗斯临床菌株中同时存在两种甚至三种不同类型的CRISPR/Cas系统的首次报告。数据包括分子分型和基因型抗生素抗性测定，以及全长基因和抗CRISPR基因序列的系统发育分析、预测的前噬菌体序列，并进行了详细的CRISPR阵列分析。研究了携带不同类型和数量CRISPR/Cas系统的分离株之间的差异。缺乏假定CRISPR/Cas系统的分离株中的毒力因子模式与具有单个或多个假定CRISPR/Cas系统的样本的毒力因子模式显著不同。我们发现具有不同CRISPR/Cas元件模式的分离株中，前噬菌体序列、抗生素抗性基因和毒力基因的数量之间存在显著相关性。我们相信所呈现的数据将有助于细菌致病适应性领域的进一步研究，包括抗菌抗性以及CRISPR/Cas系统在该基因组可塑性中的作用。
