Shelenkov Andrey, Petrova Lyudmila, Fomina Valeria, Zamyatin Mikhail, Mikhaylova Yulia, Akimkin Vasiliy
Central Research Institute of Epidemiology, Novogireevskaya str. 3a, 111123 Moscow, Russia.
National Medical and Surgical Center named after N.I. Pirogov, Nizhnyaya Pervomayskaya str., 70, 105203 Moscow, Russia.
Microorganisms. 2020 Nov 12;8(11):1775. doi: 10.3390/microorganisms8111775.
is a component of the normal intestinal microflora of humans and animals, but can cause urinary tract infections and even sepsis in hospital settings. In recent years, the number of multidrug-resistant isolates, including the ones producing extended-spectrum β-lactamases (ESBLs), is increasing worldwide. However, the number of investigations dedicated to this species, especially, whole-genome sequencing, is much lower in comparison to the members of the ESKAPE pathogens group. This study presents a detailed analysis of clinical multidrug-resistant ESBL-producing isolate using short- and long-read whole-genome sequencing, which allowed us to reveal possible horizontal gene transfer between and plasmids and to locate the CRISPR-Cas system in the genome together with its probable phage targets, as well as multiple virulence genes. We believe that the data presented will contribute to the understanding of antibiotic resistance acquisition and virulence mechanisms for this important pathogen.
它是人和动物正常肠道微生物群的一个组成部分，但在医院环境中可导致尿路感染甚至败血症。近年来，包括产超广谱β-内酰胺酶（ESBLs）菌株在内的多重耐药菌株在全球范围内不断增加。然而，与ESKAPE病原体组的成员相比，针对该物种的研究数量，尤其是全基因组测序的数量要少得多。本研究使用短读长和长读长全基因组测序对临床多重耐药产ESBLs菌株进行了详细分析，这使我们能够揭示该菌株与质粒之间可能的水平基因转移，并在基因组中定位CRISPR-Cas系统及其可能的噬菌体靶点，以及多个毒力基因。我们相信所呈现的数据将有助于理解这种重要病原体的抗生素耐药性获得和毒力机制。
