Schamberger Barbara, Plaetzer Kristjan
Laboratory of Photodynamic Inactivation of Microorganisms, Department of Biosciences, Paris Lodron University of Salzburg, 5020 Salzburg, Austria.
Morphophysics Group, Department of Chemistry and Physics of Materials, Paris Lodron University of Salzburg, 5020 Salzburg, Austria.
Antibiotics (Basel). 2021 Oct 28;10(11):1315. doi: 10.3390/antibiotics10111315.
Fungal infections in humans, contamination of food and structural damage to buildings by fungi are associated with high costs for the general public. In addition, the increase in antifungal resistance towards conventional treatment raises the demand for new fungicidal methods. Here, we present the antifungal use of Photodynamic Inactivation (PDI) based on the natural photosensitizer curcumin and a water-soluble positively charged derivative thereof (SA-CUR 12a) against two different model organisms; grown in a liquid culture and photo treated with a 435 nm LED light followed by counting of the colony-forming units and photoinactivation of tissue-like hyphal spheres of (diameter ~5 mm) with subsequent monitoring of colony growth. Curcumin (50 µM, no incubation period, i.p.) supplemented with 10% or 0.5% DMSO as well as SA-CUR 12a (50 µM no i.p or 5 min i.p.) triggered a photoantifungal effect of >4 log units towards . At 100 µM, SA-CUR 12a (0 min or 5 min i.p.) achieved a reduction of >6 log units. Colonies of shrunk significantly during PDI treatment. Photoinactivation with 50 µM or 100 µM curcumin (+0.5% DMSO) resulted in complete growth inhibition. PDI using 20, 50 or 100 µM SA-CUR 12a (with or without 10% DMSO) also showed a significant reduction in colony area compared to the control after 48 h, although less pronounced compared to curcumin. In summary, PDI using curcumin or SA-CUR 12a against or is a promising alternative to currently used fungicides, with the advantage of being very unlikely to induce resistance.
人类真菌感染、食物受真菌污染以及真菌对建筑物造成的结构破坏给公众带来了高昂成本。此外，对传统治疗的抗真菌耐药性增加，也提高了对新型杀菌方法的需求。在此，我们展示了基于天然光敏剂姜黄素及其水溶性带正电荷衍生物（SA-CUR 12a）的光动力灭活（PDI）对两种不同模式生物的抗真菌作用；在液体培养中生长，并用435 nm LED灯进行光处理，随后计数菌落形成单位，并对直径约5 mm的组织样菌丝球进行光灭活，随后监测菌落生长。姜黄素（50 μM，无孵育期，腹腔注射）添加10%或0.5%二甲基亚砜（DMSO）以及SA-CUR 12a（50 μM，无腹腔注射或5分钟腹腔注射）对[具体真菌名称未给出]引发了>4个对数单位的光抗真菌作用。在100 μM时，SA-CUR 12a（0分钟或5分钟腹腔注射）实现了>6个对数单位的减少。在PDI处理期间，[具体真菌名称未给出]的菌落显著缩小。用50 μM或100 μM姜黄素（+0.5% DMSO）进行光灭活导致完全生长抑制。使用20、50或100 μM SA-CUR 12a（有或没有10% DMSO）的PDI在48小时后与对照相比也显示出菌落面积显著减小，尽管与姜黄素相比不太明显。总之，使用姜黄素或SA-CUR 12a对[具体真菌名称未给出]进行PDI是目前使用的杀菌剂的一种有前景的替代方法，具有极不可能诱导耐药性的优点。
